Marín R, Proverbio T, Proverbio F
Biochem Pharmacol. 1985 Dec 15;34(24):4197-201. doi: 10.1016/0006-2952(85)90274-6.
Several characteristics of the Na+, K+-ATPase activity of basolateral plasma membranes of kidney proximal tubular cells from young (3 months) and old (24 months) rats were studied. In both cases, the ATPase activity reached optimum values under the following conditions: Mg2+:ATP concentrations (mM) 5:5 (apparent Km 0.5 mM); Na+ concentration 50 mM (apparent Km 18 mM); K+ concentration 20 mM (apparent Km 2.5 mM); pH 7.2; temperature 52 degrees. The values of the apparent energy of activation of the system were similar for young and old rats in the temperature range 20-52 degrees but were 55% higher for the old rats in the temperature range 10-20 degrees.
研究了幼年（3个月）和老年（24个月）大鼠肾近端小管细胞基底外侧质膜Na +，K + -ATP酶活性的几个特征。在这两种情况下，ATP酶活性在以下条件下达到最佳值：Mg2 +：ATP浓度（mM）5:5（表观Km 0.5 mM）；Na +浓度50 mM（表观Km 18 mM）；K +浓度20 mM（表观Km 2.5 mM）；pH 7.2；温度52度。在20-52度温度范围内，幼年和老年大鼠系统的表观活化能值相似，但在10-20度温度范围内，老年大鼠的表观活化能值高55％。
