Clinical Laboratory, Weifang Hospital of Traditional Chinese Medicine, Weifang, China.
Eur Rev Med Pharmacol Sci. 2018 Jul;22(1 Suppl):23-28. doi: 10.26355/eurrev_201807_15355.
We investigated the correlation between the methylation of LIVIN gene and the pathogenesis of bone tumor at the molecular level, in order to improve the treatment method and enhance the cure rate of bone tumor.
The expression level of Livin protein was detected using Western blot analysis, and its expressions in control group and patients were detected by immunohistochemistry. The methylation frequency of LIVIN gene was calculated by direct sequencing. Finally, the prognosis of treatment was investigated by follow-up.
The experiment found that Livin protein was not expressed in normal cells, while its expression rate was about 71.4% in 112 patients. The methylation frequency of LIVIN gene was gradually decreased with the increase of clinical stage, and had no significant relationship with age and sex. The prognosis experiment indicated that the lower the methylation frequency of LIVIN gene was, the shorter the survival time would be.
The methylation of LIVIN gene was closely related to the pathogenesis of bone tumor, which may be one of the important factors to induce the formation of a bone tumor. In addition, the methylation frequency of LIVIN gene could be used as a biomarker for the prognosis of bone tumor treatment.
我们从分子水平上研究 LIVIN 基因甲基化与骨肿瘤发病机制的相关性，旨在改进治疗方法，提高骨肿瘤的治愈率。
采用 Western blot 分析检测 Livin 蛋白的表达水平，免疫组织化学法检测对照组和患者组的表达情况。采用直接测序法计算 LIVIN 基因的甲基化频率。最后通过随访调查治疗的预后情况。
实验发现，Livin 蛋白在正常细胞中不表达，而在 112 例患者中的表达率约为 71.4%。LIVIN 基因的甲基化频率随临床分期的增加而逐渐降低，与年龄和性别无显著关系。预后实验表明，LIVIN 基因甲基化频率越低，生存时间越短。
LIVIN 基因的甲基化与骨肿瘤的发病机制密切相关，可能是诱导骨肿瘤形成的重要因素之一。此外，LIVIN 基因甲基化频率可作为骨肿瘤治疗预后的标志物。
