[2013～2014年中国昆明市住院婴幼儿中星状病毒感染的分子流行病学]

[Molecular Epidemiology of Astrovirus Infection Among Hospitalized Infants in Kunming City, China, 2013～2014].

作者信息

Yang Sida, Ren Yuheng, Deng Lili, Yang Jinghui, Xiong Yongying, Jing Shenrong, Li Li

出版信息

Bing Du Xue Bao. 2016 Nov;32(6):752-7.

Abstract

We investigated the infection and molecular-epidemiologic characteristics of human astrovirus (HAstV) of hospitalized infants in Kunming City from the year 2013 to 2014.Infection and genotype of HAstV of 63 samples of diarrheal feces and 42 controls were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The complete genome sequence of a HAstV strain was amplified and sequenced. The positive rate of HAstV in 63 feces samples was 41.27%(26/63).The main circulating genotype of HAstV was HAstV1.Only 1sample was positive for HAstV in 42controls(2.38%).A complete genome sequence of the HAstV strain was identified as HAstV1 by phylogenetic analyses. These data provide an important theoretical basis for the control of viral diarrhea in infants in Kunming City.

摘要

我们对2013年至2014年昆明市住院婴儿的人星状病毒（HAstV）感染情况及分子流行病学特征进行了调查。通过逆转录聚合酶链反应（RT-PCR）分析了63份腹泻粪便样本和42份对照样本中HAstV的感染情况及基因型。扩增并测序了一株HAstV毒株的全基因组序列。63份粪便样本中HAstV的阳性率为41.27%（26/63）。HAstV的主要流行基因型为HAstV1。42份对照样本中仅1份HAstV呈阳性（2.38%）。通过系统发育分析，将该HAstV毒株的全基因组序列鉴定为HAstV1。这些数据为昆明市婴儿病毒性腹泻的防控提供了重要的理论依据。