Department of Microbiology, Chiang Mai University, Chiang Mai, Thailand.
J Med Virol. 2012 Nov;84(11):1751-6. doi: 10.1002/jmv.23396.
Human astrovirus (HAstV) is one of the causative agents of acute gastroenteritis in children worldwide. The objective of this study was to elucidate the molecular epidemiology and genotypic diversity of HAstV circulating in pediatric patients admitted to hospital with diarrhea in Thailand during the year 2000-2011, except for 2004, 2006, and 2009. A total of 1,022 fecal specimens were tested for HAstV by reverse transcription polymerase chain reaction (RT-PCR). HAstV was detected at 1.4% (14 of 1,022). All HAstV strains detected in this study were characterized further by nucleotide sequencing and phylogenetic analysis. Analysis of 348 bp partial capsid nucleotide sequences revealed that HAstV strains detected were HAstV-1 (1a, 1b, and 1d) (8 strains), HAstV-2 (2c) (3 strains), HAstV-3 (1 strain), and HAstV-5 (2 strains). HAstV-1, the most predominant genotype was detected initially in 2002 and circulated continuously up to 2011. HAstV-2 was detected in year 2001, and 2007 and grouped into a 2c lineage. HAstV-3 was found only in 2000 and HAstV-5 was found in the year 2001. The findings indicate that a wide variety of HAstV strains continue to circulate in children admitted to hospital with acute gastroenteritis in Thailand over a decade. The data provide an epidemiological overview of HAstV infection and HAstV genotype distribution in Thailand.
人类星状病毒（HAstV）是导致全球儿童急性肠胃炎的病原体之一。本研究旨在阐明 2000-2011 年期间，泰国除 2004 年、2006 年和 2009 年以外，因腹泻住院的儿科患者中流行的 HAstV 的分子流行病学和基因型多样性。通过逆转录聚合酶链反应（RT-PCR）检测了 1022 份粪便标本的 HAstV。HAstV 的检出率为 1.4%（14/1022）。本研究中检测到的所有 HAstV 株均通过核苷酸测序和系统进化分析进一步进行了特征分析。对 348bp 部分衣壳核苷酸序列的分析表明，检测到的 HAstV 株为 HAstV-1（1a、1b 和 1d）（8 株）、HAstV-2（2c）（3 株）、HAstV-3（1 株）和 HAstV-5（2 株）。HAstV-1 是最主要的基因型，于 2002 年首次检出，并持续传播至 2011 年。HAstV-2 于 2001 年检出，属于 2c 谱系。HAstV-3 仅在 2000 年检出，HAstV-5 仅在 2001 年检出。研究结果表明，10 多年来，多种 HAstV 株继续在泰国因急性肠胃炎住院的儿童中传播。该数据提供了泰国 HAstV 感染和 HAstV 基因型分布的流行病学概况。
