在平行排列的 DNA 双螺旋中稳定的锌(II)介导的碱基对。
A stable zinc(II)-mediated base pair in a parallel-stranded DNA duplex.
机构信息
Westfälische Wilhelms-Universität Münster, Institut für Anorganische und Analytische Chemie, NRW Graduate School of Chemistry, Corrensstraße 28/30, 48149 Münster, Germany.
Westfälische Wilhelms-Universität Münster, Institut für Anorganische und Analytische Chemie, NRW Graduate School of Chemistry, Corrensstraße 28/30, 48149 Münster, Germany.
出版信息
J Inorg Biochem. 2018 Sep;186:301-306. doi: 10.1016/j.jinorgbio.2018.07.002. Epub 2018 Jul 5.
A stable zinc(II)-mediated base pair was formed within a parallel-stranded DNA duplex comprising a GNA (glycol nucleic acid) functionalized nucleoside analog containing the artificial nucleobase 1H-imidazo[4,5-f][1,10]phenanthroline (P). The formation of the metal-mediated base pair was confirmed by UV and CD spectroscopic analyses. In the Zn(II)-mediated homo base pairs of the type P-Zn(II)-P, the metal ion adopts a [2 + 2] coordination environment. CD spectroscopic data suggest that the chiral metal complex is formed enantiospecifically, likely induced by the helical chirality of the surrounding DNA duplex. The Zn(II)-mediated base pair stabilizes the DNA oligonucleotide duplex by 9 °C. This stable Zn(II)-mediated base pair within a parallel-stranded duplex extends the scope of site-specific functionalization of nucleic acids by virtue of metal-mediated base pairing.
在包含人工碱基 1H-咪唑并[4,5-f][1,10]菲咯啉（P）的 GNA（糖核酸）功能化核苷类似物组成的平行链 DNA 双链体中形成了稳定的锌(II)介导的碱基对。通过紫外和 CD 光谱分析证实了金属介导的碱基对的形成。在 P-Zn(II)-P 类型的锌(II)介导的同型碱基对中，金属离子采用 [2+2]配位环境。CD 光谱数据表明，手性金属配合物是通过周围 DNA 双链体的螺旋手性特异性形成的，可能是由周围 DNA 双链体的螺旋手性诱导的。锌(II)介导的碱基对通过 9°C 稳定 DNA 寡核苷酸双链体。这种在平行链双链体中的稳定锌(II)介导的碱基对通过金属介导的碱基配对扩展了核酸定点功能化的范围。
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