Bioinspired Molecular Engineering Laboratory, TechMed Centre , University of Twente , P.O. Box 217, 7500 AE Enschede , The Netherlands.
Molecular Nanofabrication Group, MESA+ Institute for Nanotechnology , University of Twente , P.O. Box 217, 7500 AE Enschede , The Netherlands.
Langmuir. 2018 Aug 7;34(31):9298-9306. doi: 10.1021/acs.langmuir.8b00853. Epub 2018 Jul 26.
Release of growth factors while simultaneously maintaining their full biological activity over a period of days to weeks is an important issue in controlled drug delivery and in tissue engineering. In addition, the selected strategy to immobilize growth factors largely determines their biological activity. Silica surfaces derivatized with glycidyloxy propyl trimethoxysilane and poly(glycidyl methacrylate) brushes yielded epoxide-functionalized surfaces onto which human bone morphogenetic protein-6 (hBMP-6) was immobilized giving stable secondary amine bonds. The biological activity of hBMP-6 was unleashed by hydrolysis of the surface siloxane and ester bonds. We demonstrate that this type of labile bonding strategy can be applied to biomaterial surfaces with relatively simple and biocompatible chemistry, such as siloxane, ester, and imine bonds. Our data indicates that the use of differential hydrolytically labile linkers is a versatile method for functionalization of biomaterials with a variety of growth factors providing control over their biological activity.
在数天到数周的时间内释放生长因子，同时保持其完全的生物活性，这是控制药物释放和组织工程中的一个重要问题。此外，固定生长因子的所选策略在很大程度上决定了它们的生物活性。用 3-缩水甘油醚氧基丙基三甲氧基硅烷和聚（甲基丙烯酸缩水甘油酯）刷衍生的二氧化硅表面得到环氧化物功能化表面，其上固定了人骨形态发生蛋白-6（hBMP-6），形成稳定的仲胺键。hBMP-6 的生物活性通过水解表面硅氧烷和酯键释放出来。我们证明，这种类型的不稳定键合策略可以应用于具有相对简单和生物相容性化学的生物材料表面，如硅氧烷、酯和亚胺键。我们的数据表明，使用差示水解不稳定的连接子是一种多功能的方法，用于用各种生长因子对生物材料进行功能化，从而控制其生物活性。
