Shameh Shahla, Hosseini Bahman, Alirezalu Abolfazl, Maleki Ramin
Faculty of Agriculture, Department of Horticultural Sciences, Urmia University, Urmia, Iran.
Iranian Academic Center for Education, Culture and Research (ACECR), Research Department of Chromatography, Urmia, Iran.
J AOAC Int. 2018 Nov 1;101(6):1788-1793. doi: 10.5740/jaoacint.18-0111. Epub 2018 Jul 13.
The petals of species are used in the food industry and various traditional medicinal products, but few studies exist on the phytochemical composition and antioxidant activity of petals of L. species grown in Iran. Phytochemical characteristics and antioxidant activity and some phenolic compounds of petals of six L. species were studied. Total phenolic content, total flavonoid content, and antioxidant activity were determined using Folin-Ciocalteu reagent, aluminum chloride method, ferric-reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, respectively. An HPLC system was used for quantitative analysis of phytochemical compounds. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) were performed among the variables analyzed using Minitab software. Also, heat maps were used to visualize phytochemical characteristics and antioxidant activity in each species using GraphPad Prism software. The amount of total phenol content, total flavonoid content, and antioxidant activity were in the range of 25.13-52.01 mg gallic acid equivalents/g dry weight (DW), 0.61-0.82 mg quercetin equivalents/g DW, 11.47-20.93 µmol Fe/g DW (FRAP), and 31.66-74.44% (DPPH), respectively. The p-coumaric acid (647.28 μg/g DW) and chlorogenic acid (24.37-135.23 μg/g DW) were found to be the most abundant phenolic compounds in the extracts of rose petals. The HCA and PCA revealed three distinct categories of species based on phytochemical composition and antioxidant activity. These results showed that phytochemical characteristics of different rose species widely correlated with species type and are promising sources of natural antioxidants beneficial for use in the food or pharmaceutical industries. Iran is one of the main centers for genetic diversity of L. The petals of species are used in the food industry and various traditional medicinal products, but few studies exist on the phytochemical composition and antioxidant activity of petals of L. species grown in Iran. Antioxidant activity and phytochemical compound of Six L. species petals grown in Iran were studied. Phenolic compounds in petals of were analyzed by HPLC. The color parameters, amount of total phenolic, total flavonoids, antioxidant activity and some individual phenolic compounds were significantly variable amongst species.
该属植物的花瓣用于食品工业和各种传统医药产品，但针对伊朗种植的该属植物花瓣的植物化学成分和抗氧化活性的研究较少。对六种该属植物花瓣的植物化学特征、抗氧化活性及一些酚类化合物进行了研究。分别采用福林-酚试剂法、氯化铝法、铁还原抗氧化能力（FRAP）法和2,2-二苯基-1-苦基肼（DPPH）法测定总酚含量、总黄酮含量和抗氧化活性。使用高效液相色谱系统对植物化学化合物进行定量分析。使用Minitab软件对分析的变量进行层次聚类分析（HCA）和主成分分析（PCA）。此外，使用GraphPad Prism软件绘制热图以直观呈现每个物种的植物化学特征和抗氧化活性。总酚含量、总黄酮含量和抗氧化活性分别在25.13 - 52.01毫克没食子酸当量/克干重（DW）、0.61 - 0.82毫克槲皮素当量/克DW、11.47 - 20.93微摩尔铁/克DW（FRAP）和31.66 - 74.44%（DPPH）范围内。对香豆酸（647.28微克/克DW）和绿原酸（24.37 - 135.23微克/克DW）被发现是玫瑰花瓣提取物中含量最丰富的酚类化合物。HCA和PCA基于植物化学成分和抗氧化活性揭示了三个不同的物种类别。这些结果表明，不同玫瑰物种的植物化学特征与物种类型广泛相关，是食品或制药行业中天然抗氧化剂的有前景来源。伊朗是该属植物遗传多样性的主要中心之一。该属植物的花瓣用于食品工业和各种传统医药产品，但针对伊朗种植的该属植物花瓣的植物化学成分和抗氧化活性的研究较少。对伊朗种植的六种该属植物花瓣的抗氧化活性和植物化学化合物进行了研究。通过高效液相色谱法分析了该属植物花瓣中的酚类化合物。该属植物物种之间的颜色参数、总酚、总黄酮含量、抗氧化活性和一些单个酚类化合物存在显著差异。
