Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria; Département de Biochimie et Microbiologie, Faculté des Sciences Biologiques et des Sciences Agronomiques, Université de Tizi ouzou, 15000, Tizi ouzou, Algeria.
Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
Phytochemistry. 2018 Oct;154:47-55. doi: 10.1016/j.phytochem.2018.06.015. Epub 2018 Jun 30.
This work aims to fill the gap in the present knowledge about the structure of pectin from Opuntia ficus-indica. The water-soluble pectin (WSP) fraction, extracted with the Microwave Assisted Extraction (MAE), was further deproteinated (dWSP) and analyzed through several biophysical and biochemical techniques. HPSEC, light scattering and FTIR data showed that dWSP is low methylated high molecular weight pectin. The biochemical structure of dWSP, after methanolysis, silylation, carboxyl reduction showed that dWSP belongs to rhamnogalacturonan I class. Then, dWSP was heat-modified (HM) to obtain small-molecular weight deproteinated fraction (HM-dWSP). Both species, dWSP and HM-dWSP, were tested in LAN5 and NIH 3T3 model cells to study their biological effect. Results indicated that both dWSP and HM-dWSP exerted cytotoxic activity affecting selectively LAN5 cancer cells, without any effect on NIH 3T3 normal cells.
本工作旨在填补目前关于 Opuntia ficus-indica 果胶结构知识的空白。采用微波辅助提取（MAE）提取的水溶性果胶（WSP）部分进一步脱蛋白（dWSP），并通过多种生物物理和生化技术进行分析。HPSEC、光散射和 FTIR 数据表明，dWSP 是低甲氧基、高分子量的果胶。经过甲醇解、硅烷化、羧基还原后的 dWSP 的生化结构表明，dWSP 属于鼠李半乳糖醛酸聚糖 I 类。然后，对 dWSP 进行热改性（HM）以获得低分子量脱蛋白部分（HM-dWSP）。对 dWSP 和 HM-dWSP 两种物质进行 LAN5 和 NIH 3T3 模型细胞测试，以研究其生物学效应。结果表明，dWSP 和 HM-dWSP 均表现出细胞毒性活性，选择性地影响 LAN5 癌细胞，而对 NIH 3T3 正常细胞没有任何影响。
