Carmier J F, Samarut J
Cell. 1986 Jan 17;44(1):159-65. doi: 10.1016/0092-8674(86)90494-0.
To determine the function of c-fps in chicken macrophages and granulocytic cells we have infected chicken bone marrow cells with retroviruses containing the v-fps oncogene. Normal chicken macrophage progenitors, M-CFCs, give rise to macrophage colonies in semisolid cultures when macrophage colony stimulating factor (M-CSF) is added into the culture medium. Upon infection with v-fps bearing retroviruses, we observed that M-CFCs were induced to develop macrophage colonies in vitro without exogenous M-CSF. This activation results from a direct effect of v-fps on the M-CFCs. No leukemic transformation was observed in the infected colonies. By comparing the effects of several retroviruses, we showed that the induction of M-CFC development is specific to v-fps containing viruses and mediated by the v-fps protein. These observations support the hypothesis that the c-fps gene is involved in the control of proliferation and/or differentiation of myeloid cells.
为了确定c-fps在鸡巨噬细胞和粒细胞中的功能，我们用含有v-fps癌基因的逆转录病毒感染了鸡骨髓细胞。正常鸡巨噬细胞祖细胞（M-CFCs）在向培养基中添加巨噬细胞集落刺激因子（M-CSF）时，能在半固体培养物中形成巨噬细胞集落。在用携带v-fps的逆转录病毒感染后，我们观察到M-CFCs在没有外源性M-CSF的情况下被诱导在体外形成巨噬细胞集落。这种激活是由v-fps对M-CFCs的直接作用引起的。在感染的集落中未观察到白血病转化。通过比较几种逆转录病毒的作用，我们表明M-CFC发育的诱导对含v-fps的病毒具有特异性，并由v-fps蛋白介导。这些观察结果支持了c-fps基因参与髓细胞增殖和/或分化控制的假说。
