Foster D A, Shibuya M, Hanafusa H
Cell. 1985 Aug;42(1):105-15. doi: 10.1016/s0092-8674(85)80106-9.
Chicken cellular-fps (c-fps) sequences were substituted for viral-fps (v-fps) sequences in two retroviral genome structures, one that expressed a c-fps gene product that was indistinguishable from the normal c-fps gene product expressed in chicken bone marrow cells, and another that expressed a gag-fps fusion protein. When c-fps gene sequences (without linked gag gene sequences) were expressed at high levels in a viral vector, no transformation of fibroblasts was detected. It was previously demonstrated that the corresponding v-fps sequences could transform fibroblasts. When the same c-fps sequences were expressed in a form linked to gag gene sequences, transformation of fibroblasts and induction of tumors were observed. The data suggest that the c-fps gene product lacks transformation potential by itself even when overexpressed and that the transformation potential of the c-fps gene can be activated by either mutation (or mutations) in the fps coding region or by fusion with viral gag gene sequences.
在两种逆转录病毒基因组结构中，鸡细胞型-fps（c-fps）序列取代了病毒型-fps（v-fps）序列，一种表达的c-fps基因产物与鸡骨髓细胞中表达的正常c-fps基因产物无法区分，另一种表达的是gag-fps融合蛋白。当c-fps基因序列（无相连的gag基因序列）在病毒载体中高水平表达时，未检测到成纤维细胞的转化。先前已证明相应的v-fps序列可转化成纤维细胞。当相同的c-fps序列以与gag基因序列相连的形式表达时，观察到了成纤维细胞的转化和肿瘤的诱导。数据表明，即使c-fps基因产物过度表达，其本身也缺乏转化潜能，并且c-fps基因的转化潜能可通过fps编码区的一个或多个突变或与病毒gag基因序列融合来激活。
