Department of Biochemistry, KGMU, Lucknow 226003, UP, India.
Department of Biochemistry, KGMU, Lucknow 226003, UP, India.
Clin Chim Acta. 2018 Oct;485:258-261. doi: 10.1016/j.cca.2018.07.021. Epub 2018 Jul 10.
Oxygen is the most mandatory component of living organism and it may at times produce highly reactive species, the free radicals, which are destructive to normal living tissues. Degenerative diseases of central nervous system (CNS) are quite common, contributing significantly to morbidity as well as mortality %. In neurodegenerative diseases such as motor neuron disease (MND), Cerebellar Ataxia (CA) and Parkinson's disease (PD), there is no direct evidence for involvement of metals and free radicals in the etiology but circumstantial evidence provides a hypothesis that alteration in metals and free radicals contribute to the pathogenesis of neurodegeneration in these disorders. The aim of the present study was to estimate free radicals cascade i.e. damage caused in terms of malondialdehyde (MDA) and defense system Superoxide dismutase (SOD) and catalase in blood and cerebro-spinal fluid (CSF) of neurodegenerative diseases (MND, CA and PD), to analyze correlation with level of free radical and the clinical variables like age, severity of diseases and duration of illness and any possibility from this clinical parameters to identify a biomarker for diagnosis of neurodegenerative diseases. The level of MDA in CSF was 0.46 ± 0.17 in case of MND, 0.49 ± 0.13 in case of CA and 0.47 ± 0.16 in case of PD as compared control group (0.22 ± 0.06) whereas in blood MDA level was 0.10 ± 0.04 in case of MND, 0.33 ± 0.41 in case of CA and 0.47 ± 0.46 in case of PD as compared control group (0.04 ± 0.03). It was found to be highly significant (p < .001). In CSF and blood both catalase activity was statistically significantly higher as compared to control group of all cases (MND, CA and PD) and SOD activity was statistically significantly lower as compared to control group of all cases. Free radical parameters in human CSF might be a novel biomarker for the early clinical identification of neurodegenerative diseases.
氧是生物体最必需的成分，它有时会产生具有高反应性的物质，即自由基，这些自由基对正常的活组织具有破坏性。中枢神经系统（CNS）的退行性疾病相当常见，对发病率和死亡率有重大影响。在运动神经元疾病（MND）、小脑共济失调（CA）和帕金森病（PD）等神经退行性疾病中，没有直接证据表明金属和自由基参与了其病因，但间接证据提供了一种假设，即金属和自由基的改变导致了这些疾病的神经退行性发生。本研究的目的是估计自由基级联反应，即通过血液和脑脊液（CSF）中的丙二醛（MDA）和超氧化物歧化酶（SOD）和过氧化氢酶来衡量的损伤，分析其与自由基水平的相关性，并与临床变量如年龄、疾病严重程度和疾病持续时间进行相关分析，以及从这些临床参数中找到一种用于诊断神经退行性疾病的生物标志物的可能性。MND 组 CSF 中 MDA 水平为 0.46±0.17，CA 组为 0.49±0.13，PD 组为 0.47±0.16，与对照组（0.22±0.06）相比，血液 MDA 水平在 MND 组为 0.10±0.04，CA 组为 0.33±0.41，PD 组为 0.47±0.46，与对照组（0.04±0.03）相比，差异有统计学意义（p<.001）。在 CSF 和血液中，所有病例（MND、CA 和 PD）的过氧化氢酶活性均显著高于对照组，所有病例的 SOD 活性均显著低于对照组。人类 CSF 中的自由基参数可能是神经退行性疾病早期临床识别的一种新的生物标志物。
