Smith G J, Kumar R K, Hristoforidis C P, Lykke A W
Cell Biol Int Rep. 1985 Dec;9(12):1115-22. doi: 10.1016/s0309-1651(85)80009-6.
The mouse lung epithelial cell strain NAL 1A has been confirmed as type 2 pneumocyte-related by immunostaining with a type 2 pneumocyte-specific antiserum. Cytoplasmic immunoreactivity with the antiserum paralleled the appearance of phospholipid-containing osmiophilic cytoplasmic inclusions, which were much more abundant in confluent cell cultures at low passage number than in exponentially growing cultures. However, phospholipid analysis indicated that NAL 1A cells were impoverished in phosphatidylglycerol as compared to lung type 2 pneumocytes. Confluent cultures of the neoplastic cell lines NAL 1AM and NUL 1 did not reveal any reactivity with the specific antiserum.
通过用2型肺细胞特异性抗血清进行免疫染色，已证实小鼠肺上皮细胞系NAL 1A与2型肺细胞相关。抗血清的细胞质免疫反应性与含磷脂的嗜锇性细胞质内含物的出现情况平行，这些内含物在低传代数的汇合细胞培养物中比在指数生长的培养物中丰富得多。然而，磷脂分析表明，与肺2型肺细胞相比，NAL 1A细胞中的磷脂酰甘油含量较低。肿瘤细胞系NAL 1AM和NUL 1的汇合培养物未显示与特异性抗血清有任何反应。
