Smith G J, Le Mesurier S M, de Montfort M L, Lykke A W
Pathology. 1984 Oct;16(4):401-5. doi: 10.3109/00313028409084730.
Cell lines which exhibit epithelial morphology with surface microvilli and inclusion bodies characteristic of type 2 pneumocytes have been derived from normal adult mouse lung by a simple procedure involving enzymatic dispersal and mechanical elimination of other cell types. One of these cell lines designated NAL 1A, examined in detail, shows features consistent with its being related to type 2 pneumocytes of mouse lung. These features include desmosomes, dense lamellar bodies as well as phospholipid profiles related to immature surface active material, the inhibition of cell growth rate by dexamethasone, and the close similarity of the cytoskeletal protein patterns of this cell line to those of a metastatic type 2 pneumocyte-related cell line of mouse lung. The cell line from normal lung demonstrated near diploid chromosome number at low passage number with some evidence of karyotype instability at high passage number.
通过一种简单的程序，即酶解分散并机械去除其他细胞类型，已从正常成年小鼠肺中获得了具有上皮形态、表面微绒毛以及Ⅱ型肺细胞特征性包涵体的细胞系。其中一个经过详细检查的细胞系命名为NAL 1A，其特征表明它与小鼠肺的Ⅱ型肺细胞有关。这些特征包括桥粒、致密板层小体以及与未成熟表面活性物质相关的磷脂谱，地塞米松对细胞生长速率的抑制作用，以及该细胞系的细胞骨架蛋白模式与小鼠肺转移性Ⅱ型肺细胞相关细胞系的细胞骨架蛋白模式极为相似。来自正常肺的细胞系在低传代次数时显示出接近二倍体的染色体数目，在高传代次数时有一些核型不稳定的迹象。
