ALA-PDT 对铜绿假单胞菌生物膜结构、毒力因子分泌和 QS 的影响。
Effects of ALA-PDT on biofilm structure, virulence factor secretion, and QS in Pseudomonas aeruginosa.
机构信息
Department of Dermatology, Daping Hospital, The Army Medical University, Chongqing 400042, China.
Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
出版信息
Photodiagnosis Photodyn Ther. 2018 Dec;24:88-94. doi: 10.1016/j.pdpdt.2018.07.005. Epub 2018 Jul 10.
AIM
To investigate the effects of ALA-PDT on biofilm structure, virulence factor secretion, and quorum sensing (QS) in Pseudomonas aeruginosa.
MATERIALS AND METHODS
We used confocal laser scanning microscopy (CLSM), an XTT assay, scanning electron microscopy (SEM), a virulence factor assay and qRT-PCR in this study.
RESULTS
The XTT assay showed that ALA-PDT significantly inhibited the growth of planktonic P. aeruginosa. CLSM and SEM showed that ALA-PDT destroyed both bacterial and biofilm structures. The virulence factor assay showed that pyocyanin and elastase secretion were significantly inhibited in the ALA-PDT groups. qRT-PCR assays demonstrated that ALA-PDT significantly reduced the mRNA expression of QS-related genes.
CONCLUSION
ALA-PDT kills planktonic and viable biofilm-associated P. aeruginosa cells, destroys biofilm structures, reduces virulence factor secretion and affects QS system gene expression.
目的
研究 ALA-PDT 对铜绿假单胞菌生物膜结构、毒力因子分泌和群体感应（QS）的影响。
材料与方法
本研究采用共聚焦激光扫描显微镜（CLSM）、XTT 检测法、扫描电子显微镜（SEM）、毒力因子检测和 qRT-PCR。
结果
XTT 检测法显示 ALA-PDT 显著抑制浮游态铜绿假单胞菌的生长。CLSM 和 SEM 显示 ALA-PDT 破坏了细菌和生物膜结构。毒力因子检测法显示，铜绿假单胞菌分泌的绿脓菌素和弹性蛋白酶明显减少。qRT-PCR 检测显示，ALA-PDT 显著降低了与 QS 相关基因的 mRNA 表达。
结论
ALA-PDT 可杀灭浮游态和存活生物膜相关铜绿假单胞菌细胞，破坏生物膜结构，减少毒力因子分泌，影响 QS 系统基因表达。
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