Department of Pharmacy, University of "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
Department of Medical, Oral and Biotechnological Sciences, University of "G. d'Annunzio" Chieti-Pescara, 66100 Chieti, Italy.
Int J Mol Sci. 2022 Jun 22;23(13):6942. doi: 10.3390/ijms23136942.
Innovative non-antibiotic compounds such as graphene oxide (GO) and light-emitting diodes (LEDs) may represent a valid strategy for managing chronic wound infections related to resistant pathogens. This study aimed to evaluate 630 nm LED and 880 nm LED ability to enhance the GO antimicrobial activity against and -resistant strains in a dual-species biofilm in the Lubbock chronic wound biofilm (LCWB) model. The effect of a 630 nm LED, alone or plus 5-aminolevulinic acid (ALAD)-mediated photodynamic therapy (PDT) (ALAD-PDT), or an 880 nm LED on the GO (50 mg/l) action was evaluated by determining the CFU/mg reductions, live/dead analysis, scanning electron microscope observation, and reactive oxygen species assay. Among the LCWBs, the best effect was obtained with GO irradiated with ALAD-PDT, with percentages of CFU/mg reduction up to 78.96% ± 0.21 and 95.17% ± 2.56 for and , respectively. The microscope images showed a reduction in the cell number and viability when treated with GO + ALAD-PDT. In addition, increased ROS production was detected. No differences were recorded when GO was irradiated with an 880 nm LED versus GO alone. The obtained results suggest that treatment with GO irradiated with ALAD-PDT represents a valid, sustainable strategy to counteract the polymicrobial colonization of chronic wounds.
创新的非抗生素化合物，如氧化石墨烯（GO）和发光二极管（LED），可能代表了一种管理与耐药病原体相关的慢性伤口感染的有效策略。本研究旨在评估 630nm LED 和 880nm LED 增强 GO 对 Lubbock 慢性伤口生物膜（LCWB）模型中双物种生物膜中 和 耐药菌株的抗菌活性的能力。通过确定 CFU/mg 减少量、死活分析、扫描电子显微镜观察和活性氧（ROS）测定，评估了单独使用 630nm LED 或联合 5-氨基酮戊酸（ALA）介导的光动力疗法（ALA-PDT）或 880nm LED 对 GO（50mg/L）作用的影响。在 LCWB 中，用 ALA-PDT 辐照的 GO 效果最好， 分别减少了 78.96%±0.21%和 95.17%±2.56%。显微镜图像显示，在用 GO+ALA-PDT 处理后，细胞数量和活力减少。此外，ROS 的产生增加。用 880nm LED 辐照 GO 与单独使用 GO 相比，没有差异。结果表明，用 ALA-PDT 辐照的 GO 治疗是一种有效的、可持续的策略，可以对抗慢性伤口的多微生物定植。
