Centre for Surgical Technologies, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
Department of Development and Regeneration, Woman and Child, Group Biomedical Sciences, KU Leuven, Leuven, Belgium.
Sci Rep. 2018 Jul 13;8(1):10604. doi: 10.1038/s41598-018-28967-w.
Currently cell therapy is considered as an experimental strategy to assist the healing process following simulated vaginal birth injury in rats, boosting the functional and morphologic recovery of pelvic floor muscles and nerves. However, the optimal administration route and dose still need to be determined. Mesangioblasts theoretically have the advantage that they can differentiate in skeletal and smooth muscle. We investigated the fate of mesoangioblasts transduced with luciferase and green fluorescent protein reporter genes (rMABs) using bioluminescence, immunofluorescence and RT-PCR in rats undergoing simulated birth injury. rMABs were injected locally, intravenously and intra-arterially (common iliacs and aorta). Intra-arterial delivery resulted in the highest amount of rMABs in the pelvic organs region and in a more homogeneous distribution over all relevant pelvic organs. Sham controls showed that the presence of the injury is important for recruitment of intra-arterially injected rMABs. Injection through the aorta or bilaterally in the common iliac arteries resulted in comparable numbers of rMABs in the pelvic organs, yet aortic injection was faster and gave less complications.
目前，细胞疗法被认为是一种实验策略，可辅助模拟阴道分娩损伤后的大鼠愈合过程，促进盆底肌肉和神经的功能和形态恢复。然而，最佳的给药途径和剂量仍需确定。中胚层细胞理论上具有分化为骨骼肌和平滑肌的优势。我们使用生物发光、免疫荧光和 RT-PCR 研究了转染荧光素酶和绿色荧光蛋白报告基因的间充质成体细胞（rMABs）在接受模拟分娩损伤的大鼠中的命运。rMABs 局部、静脉内和动脉内（髂总动脉和主动脉）注射。动脉内给药可使 rMABs 进入盆腔器官区域的数量最多，并在所有相关的盆腔器官中实现更均匀的分布。假手术对照组表明，损伤的存在对于募集动脉内注射的 rMABs 很重要。通过主动脉或双侧髂总动脉注射可使 rMABs 进入盆腔器官的数量相当，但主动脉注射更快，并发症更少。
