School of Biological Sciences; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore.
European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstrasse 1, 69117, Heidelberg, Germany.
Sci Rep. 2018 Jul 13;8(1):10646. doi: 10.1038/s41598-018-28764-5.
In animals, commensal microbes modulate various physiological functions, including behavior. While microbiota exposure is required for normal behavior in mammals, it is not known how widely this dependency is present in other animal species. We proposed the hypothesis that the microbiome has a major influence on the behavior of the vinegar fly (Drosophila melanogaster), a major invertebrate model organism. Several assays were used to test the contribution of the microbiome on some well-characterized behaviors: defensive behavior, sleep, locomotion, and courtship in microbe-bearing, control flies and two generations of germ-free animals. None of the behaviors were largely influenced by the absence of a microbiome, and the small or moderate effects were not generalizable between replicates and/or generations. These results refute the hypothesis, indicating that the Drosophila microbiome does not have a major influence over several behaviors fundamental to the animal's survival and reproduction. The impact of commensal microbes on animal behaviour may not be broadly conserved.
在动物中，共生微生物调节各种生理功能，包括行为。虽然微生物群暴露是哺乳动物正常行为所必需的，但尚不清楚这种依赖性在其他动物物种中存在的广泛程度。我们提出了一个假设，即微生物组对醋蝇（黑腹果蝇）的行为有重大影响，醋蝇是一种主要的无脊椎动物模式生物。我们使用了几种测定方法来测试微生物组对一些特征明确的行为的贡献：防御行为、睡眠、运动和交配行为，这些行为在带有微生物的对照果蝇和两代无菌动物中进行了测试。微生物组的缺失并没有对这些行为产生很大的影响，而且小的或中等的影响在重复和/或代际之间没有可推广性。这些结果反驳了假设，表明果蝇微生物组对动物生存和繁殖所必需的几种行为没有重大影响。共生微生物对动物行为的影响可能没有广泛的保守性。
