Ino Daisuke, Iino Masamitsu
Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN, Osaka, Japan.
Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2018;1791:287-292. doi: 10.1007/978-1-4939-7862-5_23.
To study the signaling mechanism of the development and maintenance of myelinating Schwann cells (SCs) in the peripheral nervous system, in vivo techniques for SC-selective gene manipulation are useful. The present protocol describes an in vivo electroporation method that allows efficient transfection of myelinating SCs in rodent sciatic nerves. This technique allows us to introduce the genes of interest into myelinating SCs by simply applying electric pulses to the sciatic nerve after plasmid DNA injection.
为了研究外周神经系统中髓鞘形成雪旺细胞（SCs）发育和维持的信号传导机制，用于SCs选择性基因操作的体内技术很有用。本方案描述了一种体内电穿孔方法，该方法可实现对啮齿动物坐骨神经中髓鞘形成SCs的高效转染。通过在注射质粒DNA后简单地对坐骨神经施加电脉冲，这项技术使我们能够将感兴趣的基因导入髓鞘形成SCs中。
