Departamento de Genética, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Departamento de Proteínas y Ácidos Nucleicos, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
PLoS One. 2020 May 29;15(5):e0233651. doi: 10.1371/journal.pone.0233651. eCollection 2020.
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors.
RNA 和核糖体从施万细胞到轴突的转移在正常和再生的周围神经中得到了证实。此前，我们已经表明，RNA 的转移依赖于肌动蛋白细胞骨架和肌球蛋白 Va。在这里，我们探讨了微管对施万细胞核内新合成的 RNA 向坐骨神经节结微绒毛运输的贡献。免疫组织化学和定量共聚焦 FRET 分析的结果表明，施万细胞来源的 RNA 与施万细胞质中的微管共定位。此外，施万细胞衍生的 RNA 的运输对诺考达唑和秋水仙素敏感，表明其依赖于微管网络的完整性。此外，编码神经元特异性蛋白的 mRNAs 是施万细胞新合成的 RNA 群体的一部分，其中一些与 KIF1B 和 KIF5B 基于微管的马达相关。
