Esmaeilnejad Bijan, Tavassoli Mousa, Samiei Awat, Hajipour Nasser
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Parasitol Res. 2018 Oct;117(10):3315-3318. doi: 10.1007/s00436-018-6007-3. Epub 2018 Jul 13.
Ovine and caprine malignant theileriosis (OCMT), a critical condition in small ruminant production, causes lethal infections. In September 2016, a total number of 400 goats of Marghoz breed (the largest population of goat breed in Iran), in northwest of Iran, were examined for approximately 25 pre-partum abortions and 7 goats' mortality for a period of 3 days. A dead goat and her aborted fetus were brought into the Urmia Veterinary Hospital for further diagnostic investigations. The microbiological assessments including direct microscopical examination of the vaginal discharges and placentome with respect to the differential staining (Gram's staining method), conventional pure culturing, fetal abomasal contents, and the liver were negative. Microscopic examinations of blood smears of the goat and the fetus revealed characteristic of Theileria spp. piroplasms and impression smear samples from goat liver and fetal spleen were positive for Theileria Koch blue bodies. DNA analysis was performed using PCR technique and specific primers derived from the nucleotide sequences of 18S rRNA gene of T. lestoquardi; following extraction from blood samples, placentome, goat liver, and spleen of the fetus. The amplified DNA was sequenced afterwards and the corresponding sequence was registered under GenBank accession number MG208059. The sequence alignment showed that the products of PCR had a homology of 99% to known T. lestoquardi sequence registered under accession numbers of KY352037.1, KC778786.1, and JQ917458.1 in the GenBank. To our knowledge, this is a report demonstrating molecular verification of T. lestoquardi transplacental transmission in a neonatal kid of Marghoz breed of goats, its feasible role in induction of perinatal deaths and abortion in goat flocks.
绵羊和山羊恶性泰勒虫病（OCMT）是小反刍动物生产中的一种关键病症，可引发致命感染。2016年9月，在伊朗西北部，对总共400只马尔戈兹品种山羊（伊朗最大的山羊品种群体）进行了为期3天的检查，这些山羊出现了约25例产前流产以及7只山羊死亡的情况。一只死山羊及其流产胎儿被送往乌尔米亚兽医医院进行进一步诊断调查。微生物学评估包括对阴道分泌物和胎盘进行直接显微镜检查（采用鉴别染色，即革兰氏染色法）、常规纯培养、胎儿皱胃内容物检查以及肝脏检查，结果均为阴性。对山羊和胎儿的血液涂片进行显微镜检查，发现了泰勒虫属梨形虫的特征，并且来自山羊肝脏和胎儿脾脏的印片样本中泰勒虫科赫蓝体呈阳性。使用PCR技术以及源自吕氏泰勒虫18S rRNA基因核苷酸序列的特异性引物进行DNA分析；样本取自血液、胎盘、山羊肝脏以及胎儿脾脏，之后对扩增的DNA进行测序，并将相应序列注册到GenBank，登录号为MG208059。序列比对显示，PCR产物与GenBank中登录号为KY352037.1、KC778786.1和JQ917458.1的已知吕氏泰勒虫序列具有99%的同源性。据我们所知，这是一份证明吕氏泰勒虫在马尔戈兹品种新生山羊中经胎盘传播、其在山羊群围产期死亡和流产诱导中可能发挥作用的分子验证报告。
