25至-25摄氏度之间，30%、40%和50%质量分数的二甲基亚砜/水溶液中标准“BES”和“三（羟甲基）甲基甘氨酸”缓冲溶液的酸解离常数和pH值。
Acid dissociation constants and pH values for standard "bes" and "tricine" buffer solutions in 30, 40, and 50 mass% dimethyl sulfoxide/water between 25 and -25 degrees C.
作者信息
Roy R N, Gibbons J J, Baker G E
出版信息
Cryobiology. 1985 Dec;22(6):589-600. doi: 10.1016/0011-2240(85)90036-7.
Information is given concerning two standard buffer solutions suitable as pH references in 30, 40, and 50 mass% dimethyl sulfoxide (DMSO)/H2O mixed solvents at subzero temperatures from -20 to 0 degrees C, with the intention of establishing a pH (designated pH*) scale. The two buffers selected were the ampholytes N,N-bis(2-hydroxyethyl)-2-aminoethane sulfonic acid ("bes") and N-tris(hydroxymethyl)methylglycine ("tricine"), and the reference standard consisted of equal molal quantities of the buffer and its respective sodium salt. The assignment of pH* values was based on measurements of the emf of cells without liquid junction of the type: Pt;H2(g,l atm) /Bes, Na Besate, NaCl / AgCl;Ag and Pt;H2(g,l atm) /Tricine, Na Tricinate, NaCl /AgCl;Ag and the pH* was derived from a determination of K2, the equilibrium constant for the dissociation process (Buffer)+/- in equilibrium with(Buffer)- + H+.
给出了两种标准缓冲溶液的相关信息，这两种缓冲溶液适用于在零下20摄氏度至0摄氏度的温度下，作为30%、40%和50%质量分数的二甲基亚砜（DMSO）/水混合溶剂中的pH参考，目的是建立一个pH（称为pH*）标度。所选的两种缓冲剂是两性电解质N,N-双（2-羟乙基）-2-氨基乙烷磺酸（“bes”）和N-三（羟甲基）甲基甘氨酸（“tricine”），参考标准由等摩尔量的缓冲剂及其相应的钠盐组成。pH值的确定基于对如下类型无液接电池电动势的测量：Pt；H2（g，1 atm）/Bes，Na Besate，NaCl / AgCl；Ag和Pt；H2（g，1 atm）/Tricine，Na Tricinate，NaCl /AgCl；Ag，并且pH是通过测定K2得出的，K2是（Buffer）+/-与（Buffer）- + H+处于平衡时解离过程的平衡常数。
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