Brambilla Marco, Martani Francesca, Bertacchi Stefano, Vitangeli Ilaria, Branduardi Paola
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy.
Yeast. 2019 Jan;36(1):23-34. doi: 10.1002/yea.3347. Epub 2018 Oct 17.
Pab1, the major poly (A) binding protein of the yeast Saccharomyces cerevisiae, is involved in many intracellular functions associated with mRNA metabolism, such as mRNA nuclear export, deadenylation, translation initiation and termination. Pab1 consists of four RNA recognition motifs (RRM), a proline-rich domain (P) and a carboxy-terminal (C) domain. Due to its modular structure, Pab1 can simultaneously interact with poly (A) tails and different proteins that regulate mRNA turnover and translation. Furthermore, Pab1 also influences cell physiology under stressful conditions by affecting the formation of quinary assemblies and stress granules, as well as by stabilizing specific mRNAs to allow translation re-initiation after stress. The main goal of this review is to correlate the structural complexity of this protein with the multiplicity of its functions.
Pab1是酿酒酵母的主要聚腺苷酸结合蛋白，参与许多与mRNA代谢相关的细胞内功能，如mRNA核输出、去腺苷酸化、翻译起始和终止。Pab1由四个RNA识别基序（RRM）、一个富含脯氨酸的结构域（P）和一个羧基末端（C）结构域组成。由于其模块化结构，Pab1可以同时与聚腺苷酸尾巴和调节mRNA周转和翻译的不同蛋白质相互作用。此外，Pab1还通过影响五元聚集体和应激颗粒的形成，以及稳定特定mRNA以允许应激后翻译重新起始，在应激条件下影响细胞生理。本综述的主要目的是将该蛋白的结构复杂性与其功能的多样性联系起来。
