从一名脊柱裂患者中生成无整合诱导多能干细胞。
Generation of integration-free induced pluripotent stem cells from a patient with spina bifida.
作者信息
Wang Hongran, Zhao Shuying, Finnell Richard H, George Timothy, Cooney Austin J
机构信息
Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States.
Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States.
出版信息
Stem Cell Res. 2018 Aug;31:27-30. doi: 10.1016/j.scr.2018.05.007. Epub 2018 May 17.
A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida.
从一名患有脊髓脊膜膨出病史的14岁女性患者身上获取了皮肤活检样本。分离出真皮成纤维细胞，并用编码OCT3/4、SOX2、KLF4和c-MYC的仙台病毒（SeV）载体进行重编程。所产生的诱导多能干细胞（iPSC）克隆NTDi4_09A没有基因组整合的重编程基因，具有稳定的正常核型并表达多能性标志物。这些iPSC在小鼠体内形成了畸胎瘤，并在体内向三个胚层的衍生物分化。这个iPSC系为研究脊柱裂患者的基因谱提供了有用的资源。
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