China Food and Drug Administration Institute of Executive Development, Beijing 100073, China; School of Government, Peking University, Beijing 100871, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Wuhan 430070, China.
Food Res Int. 2018 Sep;111:205-211. doi: 10.1016/j.foodres.2018.04.020. Epub 2018 Apr 14.
Broccoli sprouts have attracted considerable attention because of their abundant phytochemicals and high selenium accumulation capacity, which reportedly reduce the risks of different cancers and cardiovascular diseases. In this study, an untargeted metabolomics approach was developed to investigate the effect of 5 days of treatment with 100 μmol/L selenate on the metabolome of broccoli sprouts. Results showed that the total sulfur content was slightly lower and the total selenium content was significantly higher in the selenate-treated sprouts. The multivariate statistical analyses showed that serine, d-erythronolactone, melezitose and tyrosine were obviously up-regulated, but d-glyceric acid, succinic acid and citric acid were down-regulated after selenate treatment. Moreover, metabolite pathway analyses were used to support the identification of subtle but significant changes among groups of related metabolites that cannot be observed with conventional approaches. Selenate treatment influenced the metabolism of β-alanine and glutathione, as well as the biosynthesis of plant metabolite related to the precursors of glucosinolate. These results could explain why the total glucosinolate decreased after selenate treatment.
西兰花芽因其丰富的植物化学物质和高硒积累能力而备受关注，据报道，这些物质可降低不同癌症和心血管疾病的风险。在这项研究中，采用非靶向代谢组学方法研究了 100 μmol/L 硒酸盐处理 5 天对西兰花芽代谢组的影响。结果表明，硒酸盐处理的芽中总硫含量略有降低，总硒含量显著升高。多元统计分析表明，丝氨酸、d-erythronolactone、密二糖和酪氨酸明显上调，而硒酸盐处理后 d-甘油酸、琥珀酸和柠檬酸下调。此外，代谢物途径分析用于支持鉴定常规方法无法观察到的各组相关代谢物之间的细微但显著的变化。硒酸盐处理影响β-丙氨酸和谷胱甘肽的代谢，以及与类硫苷前体相关的植物代谢物的生物合成。这些结果可以解释为什么硒酸盐处理后总硫苷减少。
