Carvacho Herna Barrientos, Pérez Carmen, Zúñiga Gustavo, Mahn Andrea
Departamento de Ingeniería Química, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Estación Central, Santiago, 9170019, Chile.
J Sci Food Agric. 2014 Sep;94(12):2555-61. doi: 10.1002/jsfa.6596. Epub 2014 Mar 18.
Broccoli sprouts are a good source of secondary metabolites, exhibiting biological activity, such as polyphenols, whose concentration is affected by the exposure to exogenous elicitors. The aim of this work was to investigate the effect of sodium selenate, chitosan and methyl jasmonate, applied directly to the seeds or through irrigation, on the content and profile of phenolic compounds in broccoli sprouts. The effect on antioxidant activity was also investigated.
Methyl jasmonate and chitosan decreased antioxidant capacity. Methyl jasmonate significantly decreased total polyphenols content in comparison with control sprouts, while chitosan significantly increased it. Sodium selenate had no statistical effect on antioxidant capacity and total polyphenols concentration. The polyphenols profile in sprouts was composed by quercetin, morine, genisteine, luteoline and sinapic acid. Elicitor type and concentration affected the synthesis of these compounds. Chitosan stimulated the synthesis of quercetin, sinapic acid and morine, whereas methyl jasmonate stimulated the synthesis of luteoline. Sodium selenate had no effect on polyphenols synthesis.
The exposure of broccoli to the elicitors produced changes in the phenolic compounds profile of broccoli sprouts. Besides, the stimulation of phenolic compounds synthesis was elicitor-specific, thus opening the possibility of managing culture conditions to increase the content of a specific phenolic compound.
西兰花芽苗是次生代谢产物的良好来源，具有生物活性，如多酚，其浓度受外源诱导子暴露的影响。本研究的目的是探究直接施用于种子或通过灌溉施加的亚硒酸钠、壳聚糖和茉莉酸甲酯对西兰花芽苗中酚类化合物含量和谱的影响。同时也研究了其对抗氧化活性的影响。
茉莉酸甲酯和壳聚糖降低了抗氧化能力。与对照芽苗相比，茉莉酸甲酯显著降低了总多酚含量，而壳聚糖则显著增加了总多酚含量。亚硒酸钠对抗氧化能力和总多酚浓度无统计学影响。芽苗中的多酚谱由槲皮素、桑色素、染料木黄酮、木犀草素和芥子酸组成。诱导子类型和浓度影响这些化合物的合成。壳聚糖刺激了槲皮素、芥子酸和桑色素的合成，而茉莉酸甲酯刺激了木犀草素的合成。亚硒酸钠对多酚合成无影响。
西兰花暴露于诱导子会导致西兰花芽苗中酚类化合物谱发生变化。此外，酚类化合物合成的刺激具有诱导子特异性，因此开启了通过控制栽培条件来增加特定酚类化合物含量的可能性。
