Clinical Laboratory, First Affiliated Hospital of the University of Chinese Medicine in Guangxi, Nanning, Guangxi, China (mainland).
Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China (mainland).
Med Sci Monit. 2018 Jul 15;24:4914-4925. doi: 10.12659/MSM.908492.
BACKGROUND miR-490-3p could play vital roles in multiple cancers. However, the role of miR-490-3p in hepatocellular carcinoma (HCC) remains uncertain. In this study, we sought to explore the underlying role of miR-490-3p in HCC. MATERIAL AND METHODS In this study, we explored the clinical role of miR-490-3p in HCC via quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and The Cancer Genome Atlas (TCGA) database. Then, a meta-analysis was performed to evaluate the expression trend and diagnostic value of miR-490-3p in HCC. Furthermore, 12 miRNA prediction algorithms were applied to predict the potential target genes of miR-490-3p. The differentially expressed genes in HCC in the Gene Expression Profiling Interactive Analysis (GEPIA) database were also selected. Additionally, bioinformatics analyses were utilized to investigate the possible functions and pathways of the target genes. RESULTS miR-490-3p was clearly down-regulated in HCC based on RT-qPCR (P=0.002). Consistent with the results of RT-qPCR, miR-490 was more highly expressed in normal liver tissue than in HCC (P<0.001). Additionally, the meta-analysis confirmed the results from RT-qPCR and TCGA. Furthermore, based on the prediction algorithms and GEPIA, a total of 113 genes were selected. According to the bioinformatics analyses, we found that the most remarkably enriched functional terms included protein transport, poly(A) RNA binding, and intracellular organelle part. Additionally, the miR-490-3p target genes were significantly related to the pathways in cancer. CONCLUSIONS We found that miR-490-3p is down-regulated in HCC and is related to genes that have potential tumoral functions. However, the exact mechanism should be confirmed by functional experiments.
miR-490-3p 在多种癌症中可能发挥重要作用。然而，miR-490-3p 在肝细胞癌 (HCC) 中的作用尚不确定。在本研究中，我们试图探讨 miR-490-3p 在 HCC 中的潜在作用。
在本研究中，我们通过定量逆转录聚合酶链反应 (RT-qPCR) 和癌症基因组图谱 (TCGA) 数据库探讨了 miR-490-3p 在 HCC 中的临床作用。然后，进行荟萃分析以评估 miR-490-3p 在 HCC 中的表达趋势和诊断价值。此外，应用 12 种 miRNA 预测算法来预测 miR-490-3p 的潜在靶基因。还从基因表达谱交互式分析 (GEPIA) 数据库中选择 HCC 中差异表达的基因。此外，还进行了生物信息学分析以研究靶基因的可能功能和途径。
基于 RT-qPCR，miR-490-3p 在 HCC 中明显下调 (P=0.002)。与 RT-qPCR 的结果一致，miR-490 在正常肝组织中的表达高于 HCC (P<0.001)。荟萃分析还证实了 RT-qPCR 和 TCGA 的结果。此外，基于预测算法和 GEPIA，共选择了 113 个基因。根据生物信息学分析，我们发现最显著富集的功能术语包括蛋白质转运、多 (A) RNA 结合和细胞内细胞器部分。此外，miR-490-3p 靶基因与癌症途径显著相关。
我们发现 miR-490-3p 在 HCC 中下调，与具有潜在肿瘤功能的基因有关。然而，确切的机制应通过功能实验来证实。
