Remaley A T, Glew R H, Kuhns D B, Basford R E, Waggoner A S, Ernst L A, Pope M
Exp Parasitol. 1985 Dec;60(3):331-41. doi: 10.1016/0014-4894(85)90039-6.
We show that a purified preparation of the prominent tartrate-resistant acid phosphatase (E.C.3.1.3.2), isolated from the external surface of the intracellular parasite Leishmania donovani (promastigote form), inhibits toxic oxidative metabolite production of neutrophils. Preincubation of a neutrophil suspension (2.5 X 10(6) cells/ml) for 15 min at 37 C with 250 units (1 unit equals 1 nmole of 4-methylumbelliferyl phosphate cleaved per hr at pH 5.5) of the acid phosphatase in Krebs-Ringer phosphate buffer (pH 7.4) decreased O2 consumption, O2- production, and H2O2 production of N-formyl-methionyl-leucyl-phenylalanine (fMet-Leu-Phe)-stimulated neutrophils to 15-25% of control values. The acid phosphatase also affected concanavalin A-stimulated O2-production by neutrophils, but had no effect on the rate of phorbol myristic acetate-stimulated O2- production, chemotactic peptide binding, degranulation, or membrane depolarization. Addition of an acid phosphatase inhibitor (Complex E; (NH4)6[P2Mo18O62] X 9H2O) to suspensions of opsonized promastigotes and neutrophils resulted in a threefold or greater enhancement of O2- production. These results suggest a possible pathophysiologic role for the acid phosphatase of L. donovani promastigotes.
我们发现，从细胞内寄生虫杜氏利什曼原虫（前鞭毛体形式）外表面分离出的纯化的抗酒石酸酸性磷酸酶（E.C.3.1.3.2）制剂，可抑制中性粒细胞产生有毒的氧化代谢产物。将中性粒细胞悬液（2.5×10⁶个细胞/毫升）在37℃下与250单位（1单位等于在pH 5.5时每小时裂解的1纳摩尔4-甲基伞形酮磷酸）的酸性磷酸酶在磷酸缓冲液（pH 7.4）中预孵育15分钟，可使N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMet-Leu-Phe）刺激的中性粒细胞的耗氧量、超氧阴离子产生量和过氧化氢产生量降至对照值的15%-25%。该酸性磷酸酶也影响刀豆球蛋白A刺激的中性粒细胞超氧阴离子产生，但对佛波酯刺激的超氧阴离子产生速率、趋化肽结合、脱颗粒或膜去极化没有影响。向调理的前鞭毛体和中性粒细胞悬液中添加酸性磷酸酶抑制剂（复合物E；(NH₄)₆[P₂Mo₁₈O₆₂]·9H₂O）可使超氧阴离子产生量增加三倍或更多。这些结果提示杜氏利什曼原虫前鞭毛体酸性磷酸酶可能具有病理生理作用。
