Vigier B, Picard J Y, Campargue J, Forest M G, Heyman Y, Josso N
Mol Cell Endocrinol. 1985 Dec;43(2-3):141-50. doi: 10.1016/0303-7207(85)90077-2.
Secretion of anti-Müllerian hormone (AMH) by immature bovine Sertoli cells in primary culture was studied through a competition-type RIA employing a polyclonal antibody and 125I-labelled purified AMH. This RIA is approximately 10 times more sensitive than the solid-phase two-site monoclonal antibody-based RIA described previously. Biosynthesis and secretion of AMH by cultured Sertoli cells require approximately 48 h, are not influenced by FSH or testosterone and steadily decrease over a one-week period of culture. Cyclic AMP response to FSH stimulation is normal in cultured cells. Whether the factors responsible for the extinction of AMH production in vitro are in any way related to those operating during normal maturation, which lead to repression of AMH biosynthesis in adult Sertoli cells, is not known at the present time and deserves further study.
利用多克隆抗体和¹²⁵I标记的纯化抗苗勒管激素（AMH），通过竞争型放射免疫分析法（RIA）研究了原代培养的未成熟牛支持细胞分泌AMH的情况。这种RIA的灵敏度比先前描述的基于固相双位点单克隆抗体的RIA高约10倍。培养的支持细胞合成和分泌AMH需要约48小时，不受促卵泡激素（FSH）或睾酮的影响，并且在一周的培养期内会稳步下降。培养细胞对FSH刺激的环磷酸腺苷（cAMP）反应正常。目前尚不清楚体外导致AMH产生消失的因素是否与正常成熟过程中起作用的因素有关，正常成熟过程会导致成年支持细胞中AMH生物合成受到抑制，这值得进一步研究。
