Flytzanis C N, McMahon A P, Hough-Evans B R, Katula K S, Britten R J, Davidson E H
Dev Biol. 1985 Apr;108(2):431-42. doi: 10.1016/0012-1606(85)90046-6.
Cloned DNA was injected into the cytoplasm of unfertilized sea urchin eggs which were then fertilized and cultured in the laboratory through metamorphosis. The exogenous DNA replicated manyfold and persisted for weeks in a majority of growing larvae, as shown by hydridizing "dot blots" of the DNA of single individuals with appropriate labeled probes. After metamorphosis 5-15% of the juvenile sea urchins retained the exogenous sequences. Genomic integration of the exogenous sequence was observed in the DNA of a postmetamorphosis juvenile.
将克隆的DNA注射到未受精的海胆卵细胞质中，然后使其受精，并在实验室中培养至变态。如用适当的标记探针与单个个体的DNA进行“斑点杂交”所示，外源DNA大量复制并在大多数正在生长的幼虫中持续存在数周。变态后，5%-15%的幼年海胆保留了外源序列。在变态后幼体的DNA中观察到外源序列的基因组整合。
