McMahon A P, Flytzanis C N, Hough-Evans B R, Katula K S, Britten R J, Davidson E H
Dev Biol. 1985 Apr;108(2):420-30. doi: 10.1016/0012-1606(85)90045-4.
Cloned DNA sequences were introduced into the cytoplasm of unfertilized sea urchin eggs by a simple microinjection technique. Sperm was then added, and development allowed to proceed. If linearized plasmids are injected they form random concatenates, and during the early development of the embryos replicate repeatedly. Eukaryotic sequences are not required for replication of the exogenous DNA. Injected supercoiled DNAs neither ligate nor replicate. Both forms of exogenous DNA persist in the embryo through pluteus stage.
通过一种简单的显微注射技术，将克隆的DNA序列导入未受精的海胆卵细胞质中。然后加入精子，使发育继续进行。如果注射线性化质粒，它们会形成随机串联体，并在胚胎的早期发育过程中反复复制。外源DNA的复制不需要真核序列。注射的超螺旋DNA既不连接也不复制。两种形式的外源DNA在胚胎中一直存在到长腕幼虫阶段。
