Kiernan J A
J Histochem Cytochem. 1977 Mar;25(3):200-5. doi: 10.1177/25.3.300086.
Compounds in which olefinic linkages are accessible to aqueous reagents reduce the chloropalladite ion [PdCl4]2-, to metallic palladium. This reaction is used in a histochemical method whereby hydrophilic unsaturated lipids are stained dark brown or black. The specificity of the new method has been confirmed by means of solvent-extraction and chemical blocking procedures and by comparison with other histochemical techniques. Yellow staining of collagen, keratin and cytoplasm is probably due to attachment of the chloropalladite anion to proteins. The yellow background can be largely decolorized by treating the sections with aqueous pyridine, which forms colorless complexes with divalent palladium. A standard technique for staining with palladium is presented and the method is discussed in relation to other histochemical procedures that demonstrate unsaturated lipids.
其中烯键可被水性试剂作用的化合物能将氯钯酸根离子[PdCl₄]²⁻还原为金属钯。该反应被用于一种组织化学方法中，借此亲水性不饱和脂质被染成深棕色或黑色。新方法的特异性已通过溶剂萃取、化学阻断程序以及与其他组织化学技术的比较得以证实。胶原蛋白、角蛋白和细胞质的黄色染色可能是由于氯钯酸根阴离子与蛋白质结合所致。通过用吡啶水溶液处理切片，黄色背景可在很大程度上脱色，吡啶能与二价钯形成无色络合物。文中给出了一种钯染色的标准技术，并结合其他显示不饱和脂质的组织化学程序对该方法进行了讨论。
