Hadler W A, Silveira S R
Acta Histochem. 1978;63(2):265-70. doi: 10.1016/s0065-1281(78)80034-8.
An almost specific and highly sensitive technique to detect histochemically choline-containing lipids is proposed. This technique is based on the effect of Hg++ and phosphomolybdic ion on the choline, that yields insoluble complexes able to react with diphenylcarbazide producing a deep blue or violet stain. The specificity and the sensitivity of this technique was investigated on filter paper strips loaded with choline-containing lipids, choline-free lipids, steroids, vitamins, proteins or choline hydrochloride. The results show that the choline-containing substances react positively. The choline-free substances react negatively except calciferol and vitamin A that show weakly positive results. This technique used on tissue sections appears very suitable to histochemical purposes.
提出了一种用于组织化学检测含胆碱脂质的几乎具有特异性且高度灵敏的技术。该技术基于汞离子和磷钼酸离子对胆碱的作用，会产生能够与二苯卡巴肼反应生成深蓝色或紫色染色的不溶性复合物。在载有含胆碱脂质、不含胆碱脂质、类固醇、维生素、蛋白质或盐酸胆碱的滤纸条上研究了该技术的特异性和灵敏度。结果表明，含胆碱物质呈阳性反应。除钙化醇和维生素A呈弱阳性结果外，不含胆碱的物质呈阴性反应。在组织切片上使用该技术似乎非常适合组织化学目的。
