通过S1敏感性揭示的布氏锥虫微小外显子转录的假定调控序列。

Putative regulatory sequences for the transcription of mini-exons in Trypanosoma brucei as revealed by S1 sensitivity.

作者信息

Michiels F, Muyldermans S, Hamers R, Matthyssens G

出版信息

Gene. 1985;36(3):263-70. doi: 10.1016/0378-1119(85)90181-7.

Abstract

The 35-nucleotide (nt) mini-exon found at the 5' end of most Trypanosoma brucei mRNAs is encoded as part of a tandem 1.35-kb repeat in genomic DNA. We cloned this DNA and identified an S1-sensitive site in supercoiled plasmids containing mini-exon repeats. This site is situated on a poly(dA-dT) stretch that is variable in length in different copies of repeat. Poly(dA-dT) is capable of forming abnormal DNA helix configurations, some of which are induced by supercoiling. The S1 site may have a role in regulation of mini-exon transcription.

摘要

在大多数布氏锥虫mRNA 5'端发现的35个核苷酸（nt）的小外显子，在基因组DNA中作为1.35 kb串联重复序列的一部分进行编码。我们克隆了这段DNA，并在含有小外显子重复序列的超螺旋质粒中鉴定出一个对S1敏感的位点。该位点位于一个聚（dA-dT）片段上，该片段在不同的重复拷贝中长度可变。聚（dA-dT）能够形成异常的DNA螺旋结构，其中一些是由超螺旋诱导产生的。S1位点可能在小外显子转录调控中发挥作用。