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布氏锥虫中一个变异特异性抗原基因转录单元的推定基因。

Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei.

作者信息

Alexandre S, Guyaux M, Murphy N B, Coquelet H, Pays A, Steinert M, Pays E

机构信息

Department of Molecular Biology, University of Brussels, Belgium.

出版信息

Mol Cell Biol. 1988 Jun;8(6):2367-78. doi: 10.1128/mcb.8.6.2367-2378.1988.

DOI:10.1128/mcb.8.6.2367-2378.1988
PMID:3405209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363435/
Abstract

In a 7-kilobase (kb) sequence upstream from the 5' barren region, the Trypanosoma brucei AnTat 1.3A expression site carries two putative genes, named ESAG 2 and ESAG 3 for expression site-associated genes, as well as a copy of ESAG 1 (D.F. Cully, H.S. Ip, and G.A.M. Cross, Cell 42:173-182, 1985). At least 3 kb of this expression site exhibits a high degree of homology with the silent telomere carrying the AnTat 1.3A basic copy, whose ESAG 1 is interrupted by stop codons. Like the antigen gene, the region containing the ESAGs is transcribed only in the bloodstream forms, although transcription of 5' barren- and ESAG 2-related sequences also occurs in cultured procyclics. Analysis of steady-state and nascent transcripts suggests a continuous transcription of the whole expression site by an RNA polymerase resistant to alpha-amanitin, possibly initiating at a polymerase I-like promoter located about 17 kb upstream from the antigen gene. This polymerase seems prone to becoming inactivated upon incubation of the trypanosomes at low temperature. The putative protein encoded by ESAG 3 may carry a hydrophobic signal peptide, suggesting interaction with a membrane.

摘要

在布氏锥虫AnTat 1.3A表达位点5' 无编码区上游7千碱基（kb）的序列中，带有两个推测基因，即与表达位点相关的基因ESAG 2和ESAG 3，以及一个ESAG 1拷贝（D.F. 卡利、H.S. 伊普和G.A.M. 克罗斯，《细胞》42:173 - 182，1985年）。该表达位点至少3 kb的序列与携带AnTat 1.3A基本拷贝的沉默端粒表现出高度同源性，其ESAG 1被终止密码子打断。与抗原基因一样，包含ESAGs的区域仅在血流型中转录，尽管在培养的前循环型中也会发生5' 无编码区和ESAG 2相关序列的转录。对稳态和新生转录本的分析表明，整个表达位点由一种对α - 鹅膏蕈碱有抗性的RNA聚合酶持续转录，可能起始于位于抗原基因上游约17 kb处的类似聚合酶I的启动子。这种聚合酶在锥虫于低温下孵育时似乎易于失活。ESAG 3编码的推测蛋白可能带有一个疏水信号肽，提示其与膜有相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/7ed02184fd32/molcellb00066-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/474acd6c2059/molcellb00066-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/41f7249e8a79/molcellb00066-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/dca71f34ae9b/molcellb00066-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/a042f404f13c/molcellb00066-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/735a9214ae58/molcellb00066-0118-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/7ed02184fd32/molcellb00066-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/474acd6c2059/molcellb00066-0113-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/41f7249e8a79/molcellb00066-0116-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/dca71f34ae9b/molcellb00066-0117-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/a042f404f13c/molcellb00066-0118-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/735a9214ae58/molcellb00066-0118-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c5a/363435/7ed02184fd32/molcellb00066-0119-a.jpg

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