Zheng Jianrong, You Wei, Zheng Chuanxi, Wan Peng, Chen Jinquan, Jiang Xiaochun, Zhu Zhixiang, Zhang Zhixiong, Gong Anqi, Li Wei, Tan Jifeng, Ji Tao, Guo Wei, Zhang Shiquan
Department of Joint and Musculoskeletal Tumor, The First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518000, P.R. China.
Department of Traumatic Orthopedics, Huizhou Municipal Central Hospital, Huizhou, Guangdong 516000, P.R. China.
Oncol Lett. 2018 Aug;16(2):1849-1854. doi: 10.3892/ol.2018.8876. Epub 2018 Jun 1.
F-box proteins are essential components of the Skp-cullin-F-box complex (a type of E3 ubiquitin ligase), and participate in cell cycle and immune responses through the ubiquitin proteasome system. F-box protein 39 () belongs to the F-box family, which has been reported to be associated with cancer oncogenesis and progression. The present study aimed to investigate the role of in osteosarcoma (OS) cell proliferation and apoptosis . It was demonstrated that U-2OS cells exhibited high expression of compared with HOS and SaOS-2 osteosarcoma cells. Thus, knockdown of was performed using lentivirus-mediated short hairpin RNA (shRNA) transfection to validate the effect of in U-2OS cells. Western blotting and RT-qPCR analysis were used to confirm the efficiency of infection by analyzing the expression level of . Using Celigo-based cell counting and MTT assays, it was demonstrated that knockdown significantly reduced the rate of cell proliferation compared with control. Caspase 3/7 activity assays and fluorescence-activated cell sorting confirmed the induction of apoptosis in U-2OS cells following knockdown. In conclusion, it was demonstrated that knockdown may significantly inhibit proliferation and promote apoptosis of U-2OS cells. Thus, may serve an important role in OS progression.
F-box蛋白是Skp-泛素连接酶复合体（一种E3泛素连接酶）的重要组成部分，通过泛素蛋白酶体系统参与细胞周期和免疫反应。F-box蛋白39（）属于F-box家族，据报道其与癌症的发生和发展有关。本研究旨在探讨其在骨肉瘤（OS）细胞增殖和凋亡中的作用。结果表明，与HOS和SaOS-2骨肉瘤细胞相比，U-2OS细胞中该蛋白表达较高。因此，利用慢病毒介导的短发夹RNA（shRNA）转染技术敲低该蛋白，以验证其在U-2OS细胞中的作用。通过蛋白质免疫印迹法和RT-qPCR分析，通过检测该蛋白的表达水平来确认感染效率。使用基于Celigo的细胞计数和MTT检测，结果表明与对照组相比，敲低该蛋白显著降低了细胞增殖率。Caspase 3/7活性检测和荧光激活细胞分选证实了敲低该蛋白后U-2OS细胞凋亡的诱导。总之，结果表明敲低该蛋白可能显著抑制U-2OS细胞的增殖并促进其凋亡。因此，该蛋白可能在骨肉瘤进展中发挥重要作用。
