Yang Yang, Li Yulong, Zhao Zhenghao, Sun Ruifang, Jiang Qiuyu, Zhao Lingyu, Wang Lumin, Liu Yingxun, Wu Fei, Shi Xingmin, Huang Chen, Shao Yuan
School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
Department of Gastroenterology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, P.R. China.
Oncol Lett. 2018 Aug;16(2):1931-1936. doi: 10.3892/ol.2018.8868. Epub 2018 Jun 1.
Previous evidence has revealed that long non-coding RNAs serve important functions in numerous types of cancer when dysregulated, including in gastric cancer (GC). In the present study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was used to detect the expression of small integral membrane protein 10 like 2A (linc00086) in GC tissues and non-cancerous tissues, and the expression of linc00086 in GC cell lines was analyzed. A RT-qPCR assay was used to assess linc00086 expression levels in GC cell lines following treatment with 5-Aza-2'-deoxycytidine (5-aza-dC), which is a DNA methyltransferase inhibitor. Small interfering RNA was used to silence the expression of methyl-CpG binding protein 2 (MeCP2), and then the expression of linc00086 was detected. Linc00086 expression was revealed to be downregulated in GC tissues and GC cell lines. Furthermore, it was revealed that 5-aza-dC induced linc00086 expression in SGC-7901 and MKN45 cells, and analysis of CpG methylation by bisulfite sequencing-polymerase chain reaction demonstrated that DNA methylation may regulate the expression of linc00086. MeCP2 is involved in gene regulation by binding to methylated promoters, and it was revealed that the knockdown of the expression of MeCP2 resulted in a higher expression of linc00086. The present study revealed that DNA methylation regulate the expression of linc00086 in human GC cell lines.
先前的证据表明，长链非编码RNA在失调时在多种类型的癌症中发挥重要作用，包括胃癌（GC）。在本研究中，采用逆转录定量聚合酶链反应（RT-qPCR）分析检测胃癌组织和癌旁组织中微小整合膜蛋白10样2A（linc00086）的表达，并分析linc00086在胃癌细胞系中的表达。使用RT-qPCR测定法评估经DNA甲基转移酶抑制剂5-氮杂-2'-脱氧胞苷（5-aza-dC）处理后的胃癌细胞系中linc00086的表达水平。使用小干扰RNA沉默甲基化CpG结合蛋白2（MeCP2）的表达，然后检测linc00086的表达。结果显示，linc00086在胃癌组织和胃癌细胞系中表达下调。此外，研究发现5-aza-dC可诱导SGC-7901和MKN45细胞中linc00086的表达，亚硫酸氢盐测序-聚合酶链反应对CpG甲基化的分析表明，DNA甲基化可能调节linc00086的表达。MeCP2通过与甲基化启动子结合参与基因调控，研究发现敲低MeCP_{2}的表达会导致linc00086表达升高。本研究表明，DNA甲基化在人胃癌细胞系中调节linc00086的表达。
