微小RNA-205在肝细胞癌中表达下调，并通过直接靶向血管内皮生长因子A抑制细胞生长和转移。
MicroRNA-205 is downregulated in hepatocellular carcinoma and inhibits cell growth and metastasis via directly targeting vascular endothelial growth factor A.
作者信息
Zhao Xuya, Zhou Shi, Wang Dazhi, He Wei, Li Junxiang, Zhang Shuai
机构信息
Department of Interventional Radiology, Guizhou Cancer Hospital, Cancer Hospital of Guizhou Medical University, Guiyang, Guizhou 550003, P.R. China.
出版信息
Oncol Lett. 2018 Aug;16(2):2207-2214. doi: 10.3892/ol.2018.8933. Epub 2018 Jun 8.
Abstract
MicroRNAs (miRs) are an emerging class of non-coding, endogenous and small RNA molecules that serve important functions in tumorigenesis and development. The present study investigated the expression, functions and molecular mechanism underlying miR-205 in hepatocellular carcinoma. miR-205 was downregulated in hepatocellular carcinoma tissues and cell lines. Ectopic miR-205 expression suppressed hepatocellular carcinoma cell proliferation, migration and invasion . In addition, vascular endothelial growth factor A (VEGFA) was identified as a functional downstream target of miR-205 in hepatocellular carcinoma. Furthermore, knockdown of VEGFA revealed the same functions with miR-205 overexpression in hepatocellular carcinoma cells. These results provided evidence that miR-205 served important functions in the inhibition of hepatocellular carcinoma cells growth and metastasis via directly targeting VEGFA, which indicated that miR-205 may have therapeutic value for hepatocellular carcinoma.
摘要
微小RNA（miRs）是一类新出现的非编码、内源性小RNA分子，在肿瘤发生和发展过程中发挥着重要作用。本研究探讨了miR-205在肝细胞癌中的表达、功能及分子机制。miR-205在肝细胞癌组织和细胞系中表达下调。异位表达miR-205可抑制肝癌细胞的增殖、迁移和侵袭。此外，血管内皮生长因子A（VEGFA）被确定为miR-205在肝细胞癌中的功能性下游靶点。此外，敲低VEGFA在肝癌细胞中显示出与过表达miR-205相同的功能。这些结果证明，miR-205通过直接靶向VEGFA在抑制肝癌细胞生长和转移中发挥重要作用，这表明miR-205可能对肝细胞癌具有治疗价值。
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