一种用于淋巴毒素的快速定量测定法。

A rapid quantitative assay for lymphotoxin.

作者信息

Smith M E, Laudico R, Papermaster B W

出版信息

J Immunol Methods. 1977;14(3-4):243-51. doi: 10.1016/0022-1759(77)90134-x.

Abstract

A rapid quantitative assay for lymphotoxin was developed with the use of a mouse cultured lymphoid cell line, L1210 as the target cell. The assay produces results which are substantially in agreement with assays employing fibroblasts as targets. After incubation with lymphotoxin containing samples, target cells were labelled with [3H]thymidine and harvested with a Multiple Automated Sample Harvester (MASH). The MASH allows multiple replicates to be obtained from which the calculation of an I50 (50% inhibition) point and lymphotoxin specific activities can be performed with high statistical reliability by means of probit transformation and analysis.

摘要

利用小鼠培养的淋巴样细胞系L1210作为靶细胞，开发了一种快速定量检测淋巴毒素的方法。该检测方法产生的结果与以成纤维细胞为靶标的检测方法基本一致。用含淋巴毒素的样品孵育后，靶细胞用[3H]胸腺嘧啶核苷标记，并用多头自动样品收集器（MASH）收获。MASH可进行多次重复实验，通过概率单位转换和分析，能以高统计学可靠性计算出I50（50%抑制）点和淋巴毒素的比活性。