Trivers G, Braungart D, Leonard E J
J Immunol. 1976 Jul;117(1):130-5.
The addition of PHA to C3H mouse spleen cells in tissue culture led to the production of lymphotoxin (LT). Cytotoxicity was assayed by addition of the culture fluids to syngeneic target cells labeled with tritiated thymidine; after an incubation period of 72 hr the amount of radioactivity released into the supernatant was measured. The LT activity in unfractionated culture fluids survived lyophilization, remained unchanged for many weeks at 4 degrees C, and progressively decreased on heating at 56 degrees C for periods from 1 to 9 hr. Based on the G-200 Sephadex distribution coefficients for several preparations, the m.w. of mouse lymphotoxin was about 41,000 daltons. Lymphotoxin from three different spleen cell production runs was recovered from isoelectric focusing columns in sharply focused peaks, the pH of which ranged from 4.4 to 4.8.
在组织培养中，向C3H小鼠脾细胞添加PHA可导致产生淋巴毒素（LT）。通过将培养液添加到用氚标记胸腺嘧啶核苷标记的同基因靶细胞中来测定细胞毒性；孵育72小时后，测量释放到上清液中的放射性量。未分级培养液中的LT活性在冻干后仍能存活，在4℃下可保持数周不变，而在56℃加热1至9小时后则会逐渐降低。根据几种制剂的G-200葡聚糖凝胶分配系数，小鼠淋巴毒素的分子量约为41,000道尔顿。从等电聚焦柱中回收了来自三次不同脾细胞制备过程的淋巴毒素，形成了尖锐聚焦的峰，其pH范围为4.4至4.8。
