The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, 710049, China.
Amino Acids. 2018 Oct;50(10):1407-1414. doi: 10.1007/s00726-018-2615-6. Epub 2018 Jul 16.
Genetic background and high-salt diet are considered key factors contributing to the development of hypertension and its associated metabolic disorders. Metabolomics is an emerging powerful tool to analyze the low-molecular weight metabolites in plasma and tissue. This study integrated metabolomics and correlation network analysis to investigate the metabolic profiles of plasma and muscle of Dahl salt-sensitive (SS) rats and SS.13 rats (control) under normal and high-salt diet. The hub metabolites, which could play important roles in the metabolic changes, were identified by correlation network analysis. The results of the network analysis were further confirmed by pathway analysis and enzyme activity analysis. The results indicated a higher amino acid levels in both plasma and muscle of SS rats fed with high-salt diet. Alanine was found as a hub metabolite with the highest score of three centrality indices and also as the significant differential metabolite in plasma of SS rats after high-salt diet. Valine and lysine were found as hub metabolites and differential metabolites in muscle of SS rats after high-salt diet. Amino acid levels increased in both plasma and muscle of SS rats fed with a high salt diet. Moreover, alanine in plasma and valine and lysine in muscle as hub metabolites could play important roles in the response to high-salt diet.
遗传背景和高盐饮食被认为是导致高血压及其相关代谢紊乱的关键因素。代谢组学是一种新兴的强大工具，可用于分析血浆和组织中的低分子量代谢物。本研究将代谢组学和相关网络分析相结合，研究了正常和高盐饮食下达尔盐敏感（SS）大鼠和 SS.13 大鼠（对照）的血浆和肌肉代谢谱。通过相关网络分析鉴定出可能在代谢变化中起重要作用的枢纽代谢物。网络分析的结果进一步通过途径分析和酶活性分析得到证实。结果表明，高盐饮食组 SS 大鼠的血浆和肌肉中氨基酸水平升高。丙氨酸被发现是具有三个中心性指数最高得分的枢纽代谢物，也是高盐饮食后 SS 大鼠血浆中的显著差异代谢物。缬氨酸和赖氨酸被发现是高盐饮食后 SS 大鼠肌肉中的枢纽代谢物和差异代谢物。高盐饮食组 SS 大鼠的血浆和肌肉中氨基酸水平均升高。此外，血浆中的丙氨酸以及肌肉中的缬氨酸和赖氨酸作为枢纽代谢物可能在高盐饮食的反应中发挥重要作用。
