Department of Cardiology, First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, China.
Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an, China.
Comb Chem High Throughput Screen. 2024;27(16):2446-2453. doi: 10.2174/0113862073284196240214082904.
A high-salt diet is a leading dietary risk factor for elevated blood pressure and cardiovascular disease. Quercetin reportedly exhibits cardioprotective and antihypertensive therapeutic effects.
The objective of this study is to examine the effect of quercetin on high-salt dietinduced elevated blood pressure in Dahl salt-sensitive (SS) rats and determine the underlying molecular mechanism.
Rats of the Dahl SS and control SS-13 BN strains were separated into five groups, SS-13 BN rats fed a low-salt diet (BL group), SS-13 BN rats fed a high-salt diet (BH group), Dahl SS rats fed a low-salt diet (SL group), Dahl SS rats fed a high-salt diet (SH group), and SH rats treated with quercetin (SHQ group). Blood pressure was checked three weeks into the course of treatment, and biochemical markers in the urine and serum were examined. Additionally, western blot was done to evaluate the sirtuin 1 (SIRT1) and endothelial nitric oxide synthase (eNOS) expression levels. Immunohistochemical analysis was performed to verify SIRT1 levels.
We demonstrated that a high-salt diet elevated blood pressure in both SS-13 BN and Dahl SS rats, and quercetin supplementation alleviated the altered blood pressure. Compared with the SH group, quercetin significantly elevated the protein expression of SIRT1 and eNOS. Immunohistochemistry results further confirmed that quercetin could improve the protein expression of SIRT1.
Quercetin reduced blood pressure by enhancing the expression of SIRT1 and eNOS in Dahl SS rats fed a high-salt diet.
高盐饮食是导致血压升高和心血管疾病的主要饮食风险因素。槲皮素据称具有心脏保护和降压治疗作用。
本研究旨在探讨槲皮素对达尔盐敏感（SS）大鼠高盐饮食诱导的血压升高的影响，并确定其潜在的分子机制。
将达尔 SS 和对照 SS-13 BN 品系的大鼠分为五组，SS-13 BN 大鼠低盐饮食（BL 组）、SS-13 BN 大鼠高盐饮食（BH 组）、达尔 SS 大鼠低盐饮食（SL 组）、达尔 SS 大鼠高盐饮食（SH 组）和 SH 大鼠用槲皮素治疗（SHQ 组）。治疗三周后检查血压，检查尿液和血清中的生化标志物。此外，还进行了 Western blot 以评估沉默信息调节因子 1（SIRT1）和内皮型一氧化氮合酶（eNOS）的表达水平。进行免疫组织化学分析以验证 SIRT1 水平。
我们表明，高盐饮食会升高 SS-13 BN 和达尔 SS 大鼠的血压，而槲皮素补充可缓解血压变化。与 SH 组相比，槲皮素显著提高了 SIRT1 和 eNOS 的蛋白表达。免疫组织化学结果进一步证实，槲皮素可以改善 SIRT1 的蛋白表达。
槲皮素通过增强高盐饮食诱导的达尔 SS 大鼠 SIRT1 和 eNOS 的表达来降低血压。
