Wang Xingyun, Xu Siliang, Chen Ling, Shen Dan, Cao Yan, Tang Ranran, Wang Xing, Ji Chenbo, Li Yun, Cui Xianwei, Guo Xirong
Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital, Obsterics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, 210004, China.
Center of Clinical Reproductive Medicine, the First Affiliated Hospital of Nanjing Medical University, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, China.
Proteomics Clin Appl. 2018 Nov;12(6):e1700172. doi: 10.1002/prca.201700172. Epub 2018 Jul 30.
Peptide drugs provide promising regimes in anti-obesity treatment. In order to identify potential bioactive peptides involved in adipogenesis.
The intracellular peptides between preadipocytes and adipocytes are compared by liquid chromatography/mass spectrometry. The underlying biological function of the identified peptides are evaluated by gene ontology (GO) and pathway analysis of their precursors. To find more potential bioactive peptides, 50 peptide sequences are identified located in the functional domains with the use of the SMART and UniProt databases. Finally, the Open Targets Platform database is used to investigate the precursors related to metabolic diseases.
A total of 181 downregulated peptides and 89 upregulated peptides after differentiation (fold change > 1.5 and p-value < 0.05) are identified. The GO and pathway analysis indicate that these differentially expressed peptides play a role in adipogenesis. A total of 10 putative peptides 6 to 26 amino acids in length are identified that might have bioactive effects in adipogenesis and metabolic diseases.
On one hand, present preliminary research provides a better understanding of the intracellular peptides during adipocyte differentiation. On the other hand, it lays a foundation for discovering new peptide drugs in anti-obesity treatment.
肽类药物为抗肥胖治疗提供了有前景的方案。为了鉴定参与脂肪生成的潜在生物活性肽。
通过液相色谱/质谱比较前脂肪细胞和脂肪细胞之间的细胞内肽。通过对其前体进行基因本体论（GO）和通路分析来评估所鉴定肽的潜在生物学功能。为了找到更多潜在的生物活性肽，利用SMART和UniProt数据库鉴定了位于功能域中的50个肽序列。最后，使用开放靶点平台数据库研究与代谢疾病相关的前体。
鉴定出分化后共181个下调肽和89个上调肽（倍数变化>1.5且p值<0.05）。GO和通路分析表明这些差异表达的肽在脂肪生成中起作用。共鉴定出10个推定肽，长度为6至26个氨基酸，可能在脂肪生成和代谢疾病中具有生物活性作用。
一方面，目前的初步研究有助于更好地理解脂肪细胞分化过程中的细胞内肽。另一方面，它为发现抗肥胖治疗中的新肽类药物奠定了基础。
