Laboratoire de Chimie des Matériaux, Département de Chimie, Faculté des Sciences Exactes et Appliquées, Université d'Oran1 "Ahmed Ben Bella", B.P 1524 El-Menaouer, 31000 Oran, Algeria.
Laboratoire de Chimie des Matériaux, Département de Chimie, Faculté des Sciences Exactes et Appliquées, Université d'Oran1 "Ahmed Ben Bella", B.P 1524 El-Menaouer, 31000 Oran, Algeria.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):2149-2155. doi: 10.1016/j.ijbiomac.2018.07.058. Epub 2018 Jul 20.
In this work, an inorganic-organic nanocomposite was prepared by combining copper exchanged-magadiite (Cu-magadiite) with chitosan. The synthesis was carried out by direct dispersion of the Cu-magadiite in the chitosan matrix. The mixture obtained is shaped into beads with an average diameter of about 1-1.2 mm. These beads were then contacted with a solution of NaBH in order to reduce loaded copper ions into copper nanoparticles species. The resulting nanocomposite (Cu-NPs-magadiite/chitosan) was characterized by XRD, FTIR, SEM, TG, UV-visible DR and EDX analysis. The results show that the magadiite was completely exfoliated confirming the formation of the organic-inorganic composite. Indeed, the encapsulation of magadiite was confirmed by the SEM images, which is presented as micron free aggregates included in the cavities of a continuous polysaccharide matrix. Otherwise, they confirm also the formation of CuNPs which are probably immobilized inside the magadiite-chitosan solid matrix. The antibacterial activity against E. coli and S. aureus was highlighted by the disc inhibition method and the minimum inhibitory concentration (MIC) was determined. The CuNPs-magadiite/chitosan nanocomposite showed a very efficient bactericidal effect against both pathogen bacteria. Additionally, the MIC values obtained for nanocomposite are of 0.25 μg/L against S. aureus and of 0.50 μg/L against E. coli.
在这项工作中，通过将铜交换镁皂石（Cu-magadiite）与壳聚糖结合，制备了一种无机-有机纳米复合材料。合成是通过将 Cu-magadiite 直接分散在壳聚糖基质中进行的。得到的混合物被成型为平均直径约为 1-1.2mm 的珠粒。然后，将这些珠粒与 NaBH 溶液接触，以将负载的铜离子还原成铜纳米颗粒。所得纳米复合材料（Cu-NPs-magadiite/chitosan）通过 XRD、FTIR、SEM、TG、UV-可见 DR 和 EDX 分析进行了表征。结果表明，完全剥离了镁皂石，证实了有机-无机复合材料的形成。事实上，通过 SEM 图像证实了镁皂石的封装，其呈现为包含在连续多糖基质腔中的微米级自由聚集体。此外，它们还证实了 CuNPs 的形成，这些 CuNPs 可能被固定在镁皂石-壳聚糖固体基质内部。通过圆盘抑制法突出了对大肠杆菌和金黄色葡萄球菌的抗菌活性，并确定了最小抑菌浓度（MIC）。CuNPs-magadiite/chitosan 纳米复合材料对两种病原体细菌均表现出非常有效的杀菌作用。此外，纳米复合材料对金黄色葡萄球菌的 MIC 值为 0.25μg/L，对大肠杆菌的 MIC 值为 0.50μg/L。
