Popescu Violeta, Molea Andreia, Moldovan Marioara, Lopes Pompilia Mioara, Mazilu Moldovan Amalia, Popescu George Liviu
Physics and Chemistry Department, Technical University of Cluj-Napoca, 28 Memorandumului Str., 400114 Cluj-Napoca, Romania.
Automotive Engineering and Transports Department, Technical University of Cluj-Napoca, 28 Memorandumului Str., 400114 Cluj-Napoca, Romania.
Materials (Basel). 2021 Jun 23;14(13):3507. doi: 10.3390/ma14133507.
Amino-acids, peptides, and protein hydrolysates, together with their coordinating compounds, have various applications as fertilizers, nutritional supplements, additives, fillers, or active principles to produce hydrogels with therapeutic properties. Hydrogel-based patches can be adapted for drug, protein, or peptide delivery, and tissue healing and regeneration. These materials have the advantage of copying the contour of the wound surface, ensuring oxygenation, hydration, and at the same time protecting the surface from bacterial invasion. The aim of this paper is to describe the production of a new type of hydrogel based on whey protein isolates (WPI), whey protein hydrolysates (WPH), and gelatin. The hydrogels were obtained by utilizing a microwave-assisted method using gelatin, glycerol, WPI or WPH, copper sulfate, and water. WPH was obtained by enzymatic hydrolysis of whey protein isolates in the presence of bromelain. The hydrogel films obtained have been characterized by FT-IR and UV-VIS spectroscopy. The swelling degree and swelling kinetics have also been determined.
氨基酸、肽和蛋白质水解物及其配位化合物作为肥料、营养补充剂、添加剂、填充剂或活性成分具有多种应用，可用于生产具有治疗特性的水凝胶。基于水凝胶的贴片可用于药物、蛋白质或肽的递送以及组织愈合和再生。这些材料的优点是能够复制伤口表面的轮廓，确保氧合、水合，同时保护表面免受细菌入侵。本文的目的是描述一种基于乳清分离蛋白（WPI）、乳清蛋白水解物（WPH）和明胶的新型水凝胶的制备。通过使用明胶、甘油、WPI或WPH、硫酸铜和水的微波辅助方法获得水凝胶。WPH是在菠萝蛋白酶存在下通过乳清分离蛋白的酶促水解获得的。所获得的水凝胶薄膜已通过傅里叶变换红外光谱（FT-IR）和紫外可见光谱（UV-VIS）进行了表征。还测定了溶胀度和溶胀动力学。
