• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-210 的调节改变了果蝇昼夜节律性运动的相位，并损害了 PDF 时钟神经元的投射。

Modulation of miR-210 alters phasing of circadian locomotor activity and impairs projections of PDF clock neurons in Drosophila melanogaster.

机构信息

Department of Biology, University of Padova, Padova, Italy.

Department of Women and Children's Health, University of Padova, Padova, Italy.

出版信息

PLoS Genet. 2018 Jul 16;14(7):e1007500. doi: 10.1371/journal.pgen.1007500. eCollection 2018 Jul.

DOI:10.1371/journal.pgen.1007500
PMID:30011269
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6062148/
Abstract

Single microRNAs are usually associated with hundreds of putative target genes that can influence multiple phenotypic traits in Drosophila, ranging from development to behaviour. We investigated the function of Drosophila miR-210 in circadian behaviour by misexpressing it within circadian clock cells. Manipulation of miR-210 expression levels in the PDF (pigment dispersing factor) positive neurons affected the phase of locomotor activity, under both light-dark conditions and constant darkness. PER cyclical expression was not affected in clock neurons, however, when miR-210 was up-regulated, a dramatic alteration in the morphology of PDF ventral lateral neuron (LNv) arborisations was observed. The effect of miR-210 in shaping neuronal projections was confirmed in vitro, using a Drosophila neuronal cell line. A transcriptomic analysis revealed that miR-210 overexpression affects the expression of several genes belonging to pathways related to circadian processes, neuronal development, GTPases signal transduction and photoreception. Collectively, these data reveal the role of miR-210 in modulating circadian outputs in flies and guiding/remodelling PDF positive LNv arborisations and indicate that miR-210 may have pleiotropic effects on the clock, light perception and neuronal development.

摘要

单个 microRNAs 通常与数百个假定的靶基因相关联，这些靶基因可以影响果蝇从发育到行为的多种表型特征。我们通过在生物钟细胞内过表达 miR-210 来研究其在昼夜节律行为中的功能。在光暗条件和持续黑暗下，操纵 PDF（色素分散因子）阳性神经元中的 miR-210 表达水平会影响运动活动的相位。然而，当 miR-210 被上调时，PER 周期性表达在生物钟神经元中不受影响，但 PDF 腹外侧神经元（LNv）分支的形态发生了显著改变。使用果蝇神经元细胞系在体外证实了 miR-210 对神经元投射的影响。转录组分析显示，miR-210 过表达会影响与昼夜节律过程、神经元发育、GTPase 信号转导和光感受相关途径的几个基因的表达。总的来说，这些数据揭示了 miR-210 在调节果蝇昼夜节律输出以及指导/重塑 PDF 阳性 LNv 分支中的作用，并表明 miR-210 可能对生物钟、光感知和神经元发育具有多效性影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/b097a6845893/pgen.1007500.g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/6b2a7b969af8/pgen.1007500.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/a9d4a67aa12c/pgen.1007500.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/d6a16ece69bf/pgen.1007500.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/04a47af37eb8/pgen.1007500.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/f42b09301cb5/pgen.1007500.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/94d4c9fb9b43/pgen.1007500.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/97d678402ca7/pgen.1007500.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/22db863ad069/pgen.1007500.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/e1f5401f3cd7/pgen.1007500.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/28bd5443fc61/pgen.1007500.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/1bbcbf73fd1f/pgen.1007500.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/b097a6845893/pgen.1007500.g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/6b2a7b969af8/pgen.1007500.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/a9d4a67aa12c/pgen.1007500.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/d6a16ece69bf/pgen.1007500.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/04a47af37eb8/pgen.1007500.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/f42b09301cb5/pgen.1007500.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/94d4c9fb9b43/pgen.1007500.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/97d678402ca7/pgen.1007500.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/22db863ad069/pgen.1007500.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/e1f5401f3cd7/pgen.1007500.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/28bd5443fc61/pgen.1007500.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/1bbcbf73fd1f/pgen.1007500.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e754/6062148/b097a6845893/pgen.1007500.g012.jpg

相似文献

1
Modulation of miR-210 alters phasing of circadian locomotor activity and impairs projections of PDF clock neurons in Drosophila melanogaster.miR-210 的调节改变了果蝇昼夜节律性运动的相位，并损害了 PDF 时钟神经元的投射。
PLoS Genet. 2018 Jul 16;14(7):e1007500. doi: 10.1371/journal.pgen.1007500. eCollection 2018 Jul.
2
The Drosophila Receptor Protein Tyrosine Phosphatase LAR Is Required for Development of Circadian Pacemaker Neuron Processes That Support Rhythmic Activity in Constant Darkness But Not during Light/Dark Cycles.果蝇受体蛋白酪氨酸磷酸酶LAR是昼夜节律起搏器神经元过程发育所必需的，这些过程在持续黑暗中支持节律性活动，但在光/暗循环中则不然。
J Neurosci. 2016 Mar 30;36(13):3860-70. doi: 10.1523/JNEUROSCI.4523-15.2016.
3
MicroRNA-92a is a circadian modulator of neuronal excitability in Drosophila.miRNA-92a 是果蝇神经元兴奋性的昼夜节律调节剂。
Nat Commun. 2017 Mar 9;8:14707. doi: 10.1038/ncomms14707.
4
PDF cycling in the dorsal protocerebrum of the Drosophila brain is not necessary for circadian clock function.PDF循环在果蝇大脑背侧原脑区对于昼夜节律时钟功能并非必需。
J Biol Rhythms. 2006 Apr;21(2):104-17. doi: 10.1177/0748730405285715.
5
The ion transport peptide is a new functional clock neuropeptide in the fruit fly Drosophila melanogaster.离子转运肽是果蝇 Drosophila melanogaster 中新的功能性时钟神经肽。
J Neurosci. 2014 Jul 16;34(29):9522-36. doi: 10.1523/JNEUROSCI.0111-14.2014.
6
Drosophila free-running rhythms require intercellular communication.果蝇的自由运行节律需要细胞间通讯。
PLoS Biol. 2003 Oct;1(1):E13. doi: 10.1371/journal.pbio.0000013. Epub 2003 Sep 15.
7
Blocking synaptic transmission with tetanus toxin light chain reveals modes of neurotransmission in the PDF-positive circadian clock neurons of Drosophila melanogaster.使用破伤风毒素轻链阻断突触传递揭示了果蝇 PDF 阳性生物钟神经元中的神经传递模式。
J Insect Physiol. 2011 Sep;57(9):1290-9. doi: 10.1016/j.jinsphys.2011.06.004. Epub 2011 Jun 17.
8
Morning and evening peaks of activity rely on different clock neurons of the Drosophila brain.果蝇大脑中早晚的活动高峰依赖于不同的生物钟神经元。
Nature. 2004 Oct 14;431(7010):869-73. doi: 10.1038/nature02935.
9
Dissection of central clock function in through cell-specific CRISPR-mediated clock gene disruption.通过细胞特异性 CRISPR 介导的时钟基因敲除来剖析中枢时钟功能。
Elife. 2019 Oct 15;8:e48308. doi: 10.7554/eLife.48308.
10
Neuron-specific knockouts indicate the importance of network communication to rhythmicity.神经元特异性敲除表明网络通讯对节律性的重要性。
Elife. 2019 Oct 15;8:e48301. doi: 10.7554/eLife.48301.

引用本文的文献

1
miR-210 is essential to retinal homeostasis in fruit flies and mice.miR-210 对于果蝇和小鼠的视网膜内稳态至关重要。
Biol Direct. 2024 Oct 11;19(1):90. doi: 10.1186/s13062-024-00542-6.
2
NF-κB factors cooperate with Su(Hw)/E4F1 to balance Drosophila/human immune responses via modulating dynamic expression of miR-210.NF-κB 因子通过调节 miR-210 的动态表达与 Su(Hw)/E4F1 合作，平衡果蝇/人类的免疫反应。
Nucleic Acids Res. 2024 Jul 8;52(12):6906-6927. doi: 10.1093/nar/gkae394.
3
miR-277 regulates the phase of circadian activity-rest rhythm in .

本文引用的文献

1
MiRNA-210: A Current Overview.微小RNA-210：当前概述
Anticancer Res. 2017 Dec;37(12):6511-6521. doi: 10.21873/anticanres.12107.
2
MicroRNAs and Cancer: A Long Story for Short RNAs.微小 RNA 与癌症：短 RNA 的长篇故事。
Adv Cancer Res. 2017;135:1-24. doi: 10.1016/bs.acr.2017.06.005. Epub 2017 Aug 12.
3
MicroRNAs in Neurodegenerative Diseases.神经退行性疾病中的微小RNA
miR-277调节昼夜活动-休息节律的阶段。 （原英文文本似乎不完整，缺少具体作用对象）
Front Physiol. 2023 Nov 28;14:1082866. doi: 10.3389/fphys.2023.1082866. eCollection 2023.
4
The developmentally dynamic microRNA transcriptome of tsetse flies, vectors of animal trypanosomiasis.采采蝇（动物锥虫病的传播媒介）发育动态微小RNA转录组
Bioinform Adv. 2021 Dec 28;2(1):vbab047. doi: 10.1093/bioadv/vbab047. eCollection 2022.
5
Profiling ATM regulated genes in Drosophila at physiological condition and after ionizing radiation.在生理条件和电离辐射后，对果蝇中 ATM 调节基因进行分析。
Hereditas. 2022 Oct 21;159(1):41. doi: 10.1186/s41065-022-00254-9.
6
Photoperiod-Dependent Expression of MicroRNA in .光周期依赖性 microRNA 在 中的表达。
Int J Mol Sci. 2022 Apr 29;23(9):4935. doi: 10.3390/ijms23094935.
7
The Role of microRNAs in the Visual System.微小RNA在视觉系统中的作用。
Front Cell Dev Biol. 2022 Apr 4;10:889677. doi: 10.3389/fcell.2022.889677. eCollection 2022.
8
Abundant small RNAs in the reproductive tissues and eggs of the honey bee, Apis mellifera.大量的小 RNA 存在于蜜蜂（Apis mellifera）的生殖组织和卵中。
BMC Genomics. 2022 Apr 4;23(1):257. doi: 10.1186/s12864-022-08478-9.
9
Mesencephalic Astrocyte-Derived Neurotrophic Factor Regulates Morphology of Pigment-Dispersing Factor-Positive Clock Neurons and Circadian Neuronal Plasticity in .中脑星形胶质细胞源性神经营养因子调节色素分散因子阳性时钟神经元的形态及昼夜节律神经元可塑性
Front Physiol. 2021 Sep 27;12:705183. doi: 10.3389/fphys.2021.705183. eCollection 2021.
10
Micro RNAs and the biological clock: a target for diseases associated with a loss of circadian regulation.微小 RNA 和生物钟：与昼夜节律调节丧失相关疾病的治疗靶点。
Afr Health Sci. 2020 Dec;20(4):1887-1894. doi: 10.4314/ahs.v20i4.46.
Int Rev Cell Mol Biol. 2017;334:309-343. doi: 10.1016/bs.ircmb.2017.04.002. Epub 2017 Jun 16.
4
A step-by-step microRNA guide to cancer development and metastasis.癌症发生和转移的分步 miRNA 指南。
Cell Oncol (Dordr). 2017 Aug;40(4):303-339. doi: 10.1007/s13402-017-0341-9. Epub 2017 Jul 26.
5
MicroRNA-92a is a circadian modulator of neuronal excitability in Drosophila.miRNA-92a 是果蝇神经元兴奋性的昼夜节律调节剂。
Nat Commun. 2017 Mar 9;8:14707. doi: 10.1038/ncomms14707.
6
Molecular modulators of the circadian clock: lessons from flies and mice.生物钟的分子调节因子：来自果蝇和小鼠的经验教训。
Cell Mol Life Sci. 2017 Mar;74(6):1035-1059. doi: 10.1007/s00018-016-2378-8. Epub 2016 Sep 29.
7
Retinal expression of small non-coding RNAs in a murine model of proliferative retinopathy.增殖性视网膜病变小鼠模型中小非编码RNA的视网膜表达
Sci Rep. 2016 Sep 22;6:33947. doi: 10.1038/srep33947.
8
mir-276a strengthens Drosophila circadian rhythms by regulating timeless expression.mir-276a通过调控无时间基因的表达来增强果蝇的昼夜节律。
Proc Natl Acad Sci U S A. 2016 May 24;113(21):E2965-72. doi: 10.1073/pnas.1605837113. Epub 2016 May 9.
9
miR-124 Regulates Diverse Aspects of Rhythmic Behavior in Drosophila.miR-124调控果蝇节律行为的多个方面。
J Neurosci. 2016 Mar 23;36(12):3414-21. doi: 10.1523/JNEUROSCI.3287-15.2016.
10
miR-124 Regulates the Phase of Drosophila Circadian Locomotor Behavior.微小RNA-124调控果蝇昼夜节律性运动行为的阶段
J Neurosci. 2016 Feb 10;36(6):2007-13. doi: 10.1523/JNEUROSCI.3286-15.2016.