Mahabir Esther, Durand Stephanie, Henderson Kenneth S, Hardy Patrick
1 Comparative Medicine, Center for Molecular Medicine, University of Cologne, Cologne, Germany.
2 Charles River Laboratories, Wilmington, USA.
Lab Anim. 2019 Feb;53(1):84-88. doi: 10.1177/0023677218785929. Epub 2018 Jul 17.
We compared the efficacies of two different individually ventilated cage systems (Allentown and Tecniplast) for health monitoring (HM) of murine infectious agents using exhaust air particle (EAP) capture and real-time PCR. After three months of monitoring, both EAP capture media allowed detection of Helicobacter, Pasteurella and Entamoeba. Use of the EAP real-time PCR for HM reduces the number of mice used.
我们使用废气颗粒（EAP）捕获和实时聚合酶链反应，比较了两种不同的独立通风笼系统（阿伦敦和泰克尼普拉斯）对小鼠传染病原体进行健康监测（HM）的效果。经过三个月的监测后，两种EAP捕获介质均能检测出幽门螺杆菌、巴斯德氏菌和内阿米巴。将EAP实时聚合酶链反应用于健康监测可减少所用小鼠的数量。
