Udén A M, Hafström I, Palmblad J
J Leukoc Biol. 1986 Jan;39(1):27-35. doi: 10.1002/jlb.39.1.27.
Chemotactic substances confer a migratory pattern for neutrophil granulocytes under agarose that is characteristic for each agent. To analyse the cause of such differences, we have studied neutrophil migration and orientation with f-Met-Leu-Phe (fMLP), leukotriene B4 (LTB4), and serum as chemoattractants. When these agents were used at optimal concentrations, it was observed that cells stimulated by LTB4 did not start migration as fast and did not migrate as far as those exposed to fMLP, but they maintained a higher degree of orientation. This delay in initiation of migration and maximal degree of orientation was even more marked when serum was the chemoattractant. These migration variables were related to the generation of gradients in the agarose of fML[3H]P, arachidonic-[3H]acid (AA, of which LTB4 is a metabolite), and fluorescein. The curvilinear AA gradient was flatter and more stable than those of fML[3H]P and fluorescein, which were linear. Thus, differences in the development and shape of the gradient of chemoattractant may contribute to differences in migration kinetics.
趋化物质赋予中性粒细胞在琼脂糖下的迁移模式，每种物质都有其特征。为了分析这种差异的原因，我们研究了以甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-Met-Leu-Phe，fMLP）、白三烯B4（LTB4）和血清作为趋化因子时中性粒细胞的迁移和定向。当以最佳浓度使用这些物质时，观察到受LTB4刺激的细胞开始迁移的速度不如受fMLP刺激的细胞快，迁移的距离也不如后者远，但它们保持了更高程度的定向。当血清作为趋化因子时，迁移起始的延迟和最大定向程度更为明显。这些迁移变量与fML[3H]P、花生四烯酸-[3H]酸（AA，LTB4是其代谢产物之一）和荧光素在琼脂糖中的梯度生成有关。曲线状的AA梯度比线性的fML[3H]P和荧光素的梯度更平缓、更稳定。因此，趋化因子梯度的形成和形状差异可能导致迁移动力学的差异。
