All-Russian Institute of Plant Protection, Podbelskogo 3, 196608 St. Petersburg, Pushkin, Russia.
Novosibirsk State Agrarian University, Dobrolubova 160, 630039 Novosibirsk, Russia.
J Invertebr Pathol. 2018 Sep;157:1-3. doi: 10.1016/j.jip.2018.07.002. Epub 2018 Jul 18.
Galleria mellonella fed 3 million Nosema pyrausta spores per larva showed 0 and 5% infestation rate at 30 °C and 24 °C, respectively. N. pyrausta virulence did not increase after passage through G. mellonella for three generations. When larvae were pretreated with phenylthiourea, Bacillus thuringiensis or combination of both, infection rates were 11%, 15% and 22%, respectively. Injection of untreated and potassium hydroxide-primed spores resulted in approximately 10% and 50% infection, respectively. G. mellonella is resistant to high dosages of N. pyrausta spores, serving as a prospective model of insect resistance to microsporidia, while host immunosuppression and/or spore activation increases success of the pathogen.
在 30°C 和 24°C 下，每只幼虫喂食 300 万枚 Nosema pyrausta 孢子，感染率分别为 0 和 5%。经过三代在大蜡螟中传播后，Nosema pyrausta 的毒力没有增加。当幼虫用苯硫脲、苏云金芽孢杆菌或两者的组合预处理时，感染率分别为 11%、15%和 22%。未经处理和氢氧化钾引发的孢子注射分别导致约 10%和 50%的感染。大蜡螟对高剂量的 Nosema pyrausta 孢子具有抗性，可作为昆虫对微孢子虫抗性的潜在模型，而宿主免疫抑制和/或孢子激活增加了病原体的成功率。
