Özgör Erkay
Department of Molecular Biology and Genetics, Cyprus International University, 99258 Mersin-10, Turkey.
Cyprus Bee and Bee Products Research Centre, Cyprus International University, 99258 Mersin-10, Turkey.
Insects. 2021 Oct 19;12(10):953. doi: 10.3390/insects12100953.
The study aims to prove the possibility of colonization of and to the intestine of the greater wax moth, detect the differences of greater wax moth based on the presence of species and examine the effect of species on the phenoloxidase level of greater wax moth compared with honeybees. Each group was fed on the 1st day of the experiment with its appropriate diet containing 10 spores per insect. Each group was checked daily, and dead insects were counted. Furthermore, changes in the level of expression of the phenoloxidase-related gene after spp. treatment on the 6th, 9th and 12th days, which was detected by Q-PCR, and the mRNA level of phenoloxidase gene were measured in all experiment groups with the CFX Connect Real-Time PCR Detection System. This study shows that L. has a 66.7% mortality rate in mixed infections, a 50% mortality rate in infection, a 40% mortality rate in infection, while there is no death in . A significant difference was found in the mixed infection group compared to the single infection groups by means of and (Duncan, < 0.05). histopathology also shows that spores multiply in the epithelial cells of greater wax moth without causing any death. The increase in the mRNA level of Phenoloxidase gene in was detected (Kruskal-Wallis, < 0.05), while the mRNA level of the Phenoloxidase gene did not change in (Kruskal-Wallis, > 0.05). These findings prove that the species can colonize into the greater wax moth, which contributes to the dissemination of these species between beehives.
该研究旨在证明[具体物种1]和[具体物种2]在大蜡螟肠道内定殖的可能性，基于[具体物种]的存在检测大蜡螟的差异，并与蜜蜂相比，研究[具体物种]对大蜡螟酚氧化酶水平的影响。在实验的第1天，给每组喂食含有每只昆虫10个孢子的适当饲料。每天检查每组，统计死亡昆虫数量。此外，通过Q-PCR检测在第6、9和12天[具体物种]处理后酚氧化酶相关基因的表达水平变化，并使用CFX Connect实时PCR检测系统测量所有实验组中酚氧化酶基因的mRNA水平。本研究表明，在混合[具体物种1]感染中，[具体物种2]的死亡率为66.7%，在[具体物种1]感染中死亡率为50%，在[具体物种3]感染中死亡率为40%，而在[具体物种4]感染中无死亡情况。通过[具体分析方法1]和[具体分析方法2]（邓肯检验，P<0.05）发现，混合[具体物种1]感染组与单一[具体物种1]感染组相比存在显著差异。[具体物种1]的组织病理学也表明，[具体物种1]孢子在大蜡螟上皮细胞中繁殖而不导致任何死亡。检测到[具体物种2]中酚氧化酶基因的mRNA水平升高（克鲁斯卡尔-沃利斯检验，P<0.05），而在[具体物种3]中酚氧化酶基因的mRNA水平没有变化（克鲁斯卡尔-沃利斯检验，P>0.05）。这些发现证明，[具体物种]能够定殖于大蜡螟体内，这有助于这些[具体物种]在蜂箱之间传播。
