Gunawan Erin Ryantin, Suhendra Dedy, Hidayat Ipan, Kurniawati Lely
Chemistry Department, Faculty of Mathematics and Science, University of Mataram.
J Oleo Sci. 2018 Aug 1;67(8):949-955. doi: 10.5650/jos.ess18042. Epub 2018 Jul 17.
Alkyldiethanolamides (fatty acid diethanolamides) synthesis from Terminalia catappa L. kernel oil was optimized using lypozyme as a catalyst. The result showed that the optimal reaction conditions were 2 hours reaction time, with a ratio of oil mass (g) to diethanolamine (mmol) of 1:5, a ratio of oil mass to enzyme (g) of 1: 0.075, and a temperature of 40°C. The percentage of alkyldiethanolamides at optimum condition was 56-60%. The synthesis results were also analyzed by FTIR. FTIR spectra revealed specific absorption at several wave numbers (3434 cm, 1655 cm, 1280 cm), indicating that amide and alcohol bonds (C=O, C-N, and O-H) were formed. GC-MS was employed to identify the types of fatty acid diethanolamides that were successfully synthesized. The fatty acid diethanolamides formed were palmitoyldiethanolamide (R = 32.96 min) and oleyldiethanolamide (R = 35.57 min). The total nitrogen content of alkyldietanolamides was 0.26%, or 0.19 mmol of the amide group in 1 g of sample.
以 Terminalia catappa L. 核仁油为原料，使用脂肪酶作为催化剂优化合成烷基二乙醇酰胺（脂肪酸二乙醇酰胺）。结果表明，最佳反应条件为反应时间2小时，油质量（g）与二乙醇胺（mmol）的比例为1:5，油质量与酶（g）的比例为1:0.075，温度为40°C。在最佳条件下烷基二乙醇酰胺的百分比为56 - 60%。还通过傅里叶变换红外光谱（FTIR）对合成结果进行了分析。FTIR光谱在几个波数（3434 cm、1655 cm、1280 cm）处显示出特定吸收，表明形成了酰胺键和醇键（C=O、C-N和O-H）。采用气相色谱 - 质谱联用（GC-MS）鉴定成功合成的脂肪酸二乙醇酰胺的类型。形成的脂肪酸二乙醇酰胺为棕榈酰二乙醇酰胺（保留时间R = 32.96分钟）和油酰二乙醇酰胺（保留时间R = 35.57分钟）。烷基二乙醇酰胺的总氮含量为0.26%，即1 g样品中含有0.19 mmol的酰胺基团。
